{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T17:27:07Z","timestamp":1773682027509,"version":"3.50.1"},"reference-count":52,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2023,4,26]],"date-time":"2023-04-26T00:00:00Z","timestamp":1682467200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Adv Synth Catal"],"published-print":{"date-parts":[[2023,5,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Although optical pure amino alcohols are in high demand due to their widespread applicability, they still remain challenging to synthesize, since commonly elaborated protection strategies are required. Here, a multi\u2010enzymatic methodology is presented that circumvents this obstacle furnishing enantioenriched 1,3\u2010amino alcohols out of commodity chemicals. A Type I aldolase forged the carbon backbone with an enantioenriched aldol motif, which was subsequently subjected to enzymatic transamination. A panel of 194 TAs was tested on diverse nine aldol products prepared through different nucleophiles and electrophiles. Due to the availability of (<jats:italic>R<\/jats:italic>)\u2010 and (<jats:italic>S<\/jats:italic>)\u2010selective TAs, both diastereomers of the 1,3\u2010amino alcohol motif were accessible. A two\u2010step process enabled the synthesis of the desired amino alcohols with up to three chiral centers with <jats:italic>de<\/jats:italic> up to &gt;97 in the final products.<\/jats:p><jats:p><jats:boxed-text content-type=\"graphic\" position=\"anchor\"><jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/adsc202300201-toc-0001-m.png\"><jats:alt-text>magnified image<\/jats:alt-text><\/jats:graphic><\/jats:boxed-text>\n<\/jats:p>","DOI":"10.1002\/adsc.202300201","type":"journal-article","created":{"date-parts":[[2023,4,12]],"date-time":"2023-04-12T22:50:20Z","timestamp":1681339820000},"page":"1485-1495","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Biocatalytic Transamination of Aldolase\u2010Derived 3\u2010Hydroxy Ketones"],"prefix":"10.1002","volume":"365","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5207-3664","authenticated-orcid":false,"given":"Mathias","family":"Pickl","sequence":"first","affiliation":[{"name":"Department of Chemical Biology Instituto de Qu\u00edmica Avanzada de Catalu\u00f1a (IQAC-CSIC)  Jordi Girona 18-26 08034 Barcelona Spain"},{"name":"Institute of Chemistry University of Graz  Heinrichstrasse 28 8010 Graz Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Markus","family":"Ebner","sequence":"additional","affiliation":[{"name":"Institute of Chemistry University of Graz  Heinrichstrasse 28 8010 Graz Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samantha","family":"Gittings","sequence":"additional","affiliation":[{"name":"Prozomix Ltd. West End Industrial Estate  Haltwhistle Northumberland NE49 9HA U.K"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5541-4794","authenticated-orcid":false,"given":"Pere","family":"Clap\u00e9s","sequence":"additional","affiliation":[{"name":"Department of Chemical Biology Instituto de Qu\u00edmica Avanzada de Catalu\u00f1a (IQAC-CSIC)  Jordi Girona 18-26 08034 Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2151-6394","authenticated-orcid":false,"given":"Wolfgang","family":"Kroutil","sequence":"additional","affiliation":[{"name":"Institute of Chemistry University of Graz  Heinrichstrasse 28 8010 Graz Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,4,26]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.7b00033"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2017.05.023"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1039\/D1CB00080B"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41929-017-0010-4"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/cs400930v"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aay8484"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/acscatal.6b02979"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2014.02.021"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr100299p"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/acscatal.6b00758"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2005.10.008"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/cctc.201200709"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cogsc.2021.100476"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/acscatal.1c04273"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/adsc.201801530"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2004-829578"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2016.11.022"},{"key":"e_1_2_6_19_1","first-page":"2251","volume":"2","author":"Ford G. J.","year":"2022","journal-title":"J. Am. Chem. Soc."},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2017.09.008"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1039\/C6GC02328B"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2017.12.007"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/acscatal.7b02686"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr050065q"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1021\/ar700100e"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.R800012-JLR200"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.orglett.7b01471"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1039\/D2CC01963A"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja026292g"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja0363462"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1021\/ol026456y"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.9b05221"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja9054959"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/adsc.201500214"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202116344"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.26470"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/cctc.201901756"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1021\/acscatal.6b03181"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1021\/acscatal.1c00210"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1271\/bbb.60655"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2007.00783.x"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1039\/C7CC00742F"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1021\/acscatal.8b02486"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/chem.200802532"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201804831"},{"key":"e_1_2_6_45_2","doi-asserted-by":"publisher","DOI":"10.1002\/ange.201804831"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.447"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-005-0002-1"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1002\/adsc.201100558"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/chem.201403281"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200902065"},{"key":"e_1_2_6_50_2","doi-asserted-by":"publisher","DOI":"10.1002\/ange.200902065"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1039\/c1cc11069a"}],"container-title":["Advanced Synthesis &amp; Catalysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adsc.202300201","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T15:59:15Z","timestamp":1759766355000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/adsc.202300201"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,4,26]]},"references-count":52,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2023,5,12]]}},"alternative-id":["10.1002\/adsc.202300201"],"URL":"https:\/\/doi.org\/10.1002\/adsc.202300201","archive":["Portico"],"relation":{},"ISSN":["1615-4150","1615-4169"],"issn-type":[{"value":"1615-4150","type":"print"},{"value":"1615-4169","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,4,26]]},"assertion":[{"value":"2023-03-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-04-26","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}