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Here, an unconventional approach is presented to restore the susceptibility of methicillin\u2010resistant\n                    <jats:italic>S. aureus<\/jats:italic>\n                    (MRSA) to a broad spectrum of conventional antibiotics via photo\u2010disassembly of functional membrane microdomains. The photo\u2010disassembly of microdomains is based on effective photolysis of staphyloxanthin, the golden carotenoid pigment that gives its name. Upon pulsed laser treatment, cell membranes are found severely disorganized and malfunctioned to defense antibiotics, as unveiled by membrane permeabilization, membrane fluidification, and detachment of membrane protein, PBP2a. Consequently, the photolysis approach increases susceptibility and inhibits development of resistance to a broad spectrum of antibiotics including penicillins, quinolones, tetracyclines, aminoglycosides, lipopeptides, and oxazolidinones. The synergistic therapy, without phototoxicity to the host, is effective in combating MRSA both in vitro and in vivo in a mice skin infection model. Collectively, this endogenous chromophore\u2010targeted phototherapy concept paves a novel platform to revive conventional antibiotics to combat drug\u2010resistant\n                    <jats:italic>S. aureus<\/jats:italic>\n                    infections as well as to screen new lead compounds.\n                  <\/jats:p>","DOI":"10.1002\/advs.201903117","type":"journal-article","created":{"date-parts":[[2020,1,27]],"date-time":"2020-01-27T09:41:07Z","timestamp":1580118067000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":44,"title":["Photo\u2010Disassembly of Membrane Microdomains Revives Conventional Antibiotics against MRSA"],"prefix":"10.1002","volume":"7","author":[{"given":"Jie","family":"Hui","sequence":"first","affiliation":[{"name":"Department of Electrical and Computer Engineering Boston University  Boston MA 02215 USA"},{"name":"Boston University Photonics Center  Boston MA 02215 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pu\u2010Ting","family":"Dong","sequence":"additional","affiliation":[{"name":"Boston University Photonics Center  Boston MA 02215 USA"},{"name":"Department of Chemistry Boston University  Boston MA 02215 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lijia","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Electrical and Computer Engineering Boston University  Boston MA 02215 USA"},{"name":"State Key Laboratory of Supramolecular Structure and Materials Institute of Theoretical Chemistry Jilin University  Changchun 130012 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taraknath","family":"Mandal","sequence":"additional","affiliation":[{"name":"Department of Chemistry Boston University  Boston MA 02215 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junjie","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Electrical and Computer Engineering Boston University  Boston MA 02215 USA"},{"name":"Boston University Photonics Center  Boston MA 02215 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erlinda R.","family":"Ulloa","sequence":"additional","affiliation":[{"name":"Collaborative to Halt Antibiotic\u2010Resistant Microbes (CHARM) Department of Pediatrics University of California San Diego School of Medicine  La Jolla CA 92093 USA"},{"name":"Division of Infectious Disease Department of Pediatrics Children's Hospital of Philadelphia  Philadelphia PA 19104 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuewei","family":"Zhan","sequence":"additional","affiliation":[{"name":"Department Biomedical Engineering Boston University  Boston MA 02215 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sebastian","family":"Jusuf","sequence":"additional","affiliation":[{"name":"Department Biomedical Engineering Boston University  Boston MA 02215 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheng","family":"Zong","sequence":"additional","affiliation":[{"name":"Department of Electrical and Computer Engineering Boston University  Boston MA 02215 USA"},{"name":"Boston University Photonics Center  Boston MA 02215 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamed N.","family":"Seleem","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Purdue University  West Lafayette IN 47907 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"George Y.","family":"Liu","sequence":"additional","affiliation":[{"name":"Collaborative to Halt Antibiotic\u2010Resistant Microbes (CHARM) Department of Pediatrics University of California San Diego School of Medicine  La Jolla CA 92093 USA"},{"name":"Division of Infectious Diseases Rady Children's Hospital  San Diego CA 92123 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiang","family":"Cui","sequence":"additional","affiliation":[{"name":"Department of Chemistry Boston University  Boston MA 02215 USA"},{"name":"Department Biomedical Engineering Boston University  Boston MA 02215 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5607-6683","authenticated-orcid":false,"given":"Ji\u2010Xin","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Electrical and Computer Engineering Boston University  Boston MA 02215 USA"},{"name":"Boston University Photonics Center  Boston MA 02215 USA"},{"name":"Department of Chemistry Boston University  Boston MA 02215 USA"},{"name":"Department Biomedical Engineering Boston University  Boston MA 02215 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,1,27]]},"reference":[{"key":"e_1_2_7_1_1","unstructured":"E. 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