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Here, a robust organoid engineering approach, Multi\u2010Organoid Patterning and Fusion (MOrPF), is presented to assemble individual airway organoids of different sizes into upscaled, scaffold\u2010free airway tubes with predefined shapes. Multi\u2010Organoid Aggregates (MOAs) undergo accelerated fusion in a matrix\u2010depleted, free\u2010floating environment, possess a continuous lumen, and maintain prescribed shapes without an exogenous scaffold interface. MOAs in the floating culture exhibit a well\u2010defined three\u2010stage process of inter\u2010organoid surface integration, luminal material clearance, and lumina connection. The observed shape stability of patterned MOAs is confirmed by theoretical modelling based on organoid morphology and the physical forces involved in organoid fusion. Immunofluorescent characterization shows that fused MOA tubes possess an unstratified epithelium consisting mainly of tracheal basal stem cells. By generating large, shape\u2010controllable organ tubes, MOrPF enables upscaled organoid engineering towards integrated organoid devices and structurally complex organ tubes.<\/jats:p>","DOI":"10.1002\/advs.202003332","type":"journal-article","created":{"date-parts":[[2021,2,9]],"date-time":"2021-02-09T06:46:07Z","timestamp":1612853167000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Bio\u2010assembling Macro\u2010Scale, Lumenized Airway Tubes of Defined Shape via Multi\u2010Organoid Patterning and Fusion"],"prefix":"10.1002","volume":"8","author":[{"given":"Ye","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Engineering University of Cambridge  Cambridge CB2 1PZ UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Dabrowska","sequence":"additional","affiliation":[{"name":"Wellcome \u2013 MRC Cambridge Stem Cell Institute University of Cambridge  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