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However, little is known about the behavior of mitochondria during oncosis, due to the lack of probes for in situ visual illumination of the mitochondrial membrane and mtDNA. Herein, a mitochondrial lipid and mtDNA dual\u2010labeled probe, <jats:bold>MitoMN<\/jats:bold>, and a continuous add\u2010on assay, are designed to image the dynamic process of mitochondria in conditions that are unobservable with current mitochondrial probes. Meanwhile, the <jats:bold>MitoMN<\/jats:bold> can induce oncosis in a light\u2010activated manner, which results in the enlargement of mitochondria and the death of cancer cells. Using structured illumination microscopy (SIM), <jats:bold>MitoMN<\/jats:bold>\u2010stained mitochondria with a dual\u2010color response reveals, for the first time, how swelled mitochondria interacts and fuses with each other for a nonlinear enlargement to accelerate oncosis into an irreversible stage. With this sign of irreversible oncosis revealed by <jats:bold>MitoMN<\/jats:bold>, oncosis can be segregated into three stages, including before oncosis, initial oncosis, and accelerated oncosis.<\/jats:p>","DOI":"10.1002\/advs.202004566","type":"journal-article","created":{"date-parts":[[2021,7,2]],"date-time":"2021-07-02T00:14:00Z","timestamp":1625184840000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["A Continuous Add\u2010On Probe Reveals the Nonlinear Enlargement of Mitochondria in Light\u2010Activated Oncosis"],"prefix":"10.1002","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0835-8803","authenticated-orcid":false,"given":"Kang\u2010Nan","family":"Wang","sequence":"first","affiliation":[{"name":"MOE Key Laboratory of Bioinorganic and Synthetic Chemistry School of Chemistry State Key Laboratory of Oncology in South China Sun Yat\u2010Sen University  Guangzhou 510275 P. R. 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