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Specifically, mucin deficiency is reported in a subset of DED patients, but its effects on ocular interfacial properties remain unclear. Herein a novel in vitro mucin\u2010deficient mimetic ocular surface (Mu\u2010DeMOS) with a controllable amount of membrane\u2010tethered mucin molecules is developed to represent the diseased ocular surfaces. Contact angle goniometry on mimetic ocular surfaces reveals that high surface roughness, but not the presence of hydrophilic mucin molecules, delivers constant hydration over native ocular surface epithelia. Live\u2010cell rheometry confirms that the presence of mucin\u2010like glycoproteins on ocular epithelial cells reduces shear adhesive strength at cellular interfaces. Together, optimal surface roughness and surface chemistry facilitate sustainable lubrication for healthy ocular surfaces, while an imbalance between them contributes to lubrication\u2010related dysfunction at diseased ocular epithelial surfaces. Furthermore, the restoration of low adhesive strength at Mu\u2010DeMOS interfaces through a mucin\u2010like glycoprotein, recombinant human lubricin, suggests that increased frictional damage at mucin\u2010deficient cellular surfaces may be reversible. More broadly, these results demonstrate that Mu\u2010DeMOS is a promising platform for drug screening assays and fundamental studies on ocular physiology.<\/jats:p>","DOI":"10.1002\/advs.202100841","type":"journal-article","created":{"date-parts":[[2021,6,29]],"date-time":"2021-06-29T08:00:23Z","timestamp":1624953623000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Mucin\u2010Like Glycoproteins Modulate Interfacial Properties of a Mimetic Ocular Epithelial Surface"],"prefix":"10.1002","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3469-3904","authenticated-orcid":false,"given":"Chunzi","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Chemical Engineering Stanford University  Stanford CA 94305 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amy C.","family":"Madl","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering Stanford University  Stanford CA 94305 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Cirera\u2010Salinas","sequence":"additional","affiliation":[{"name":"Global Drug Development Biopharmaceutical Process &amp; 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