{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T17:56:09Z","timestamp":1792173369676,"version":"4.4.0"},"reference-count":56,"publisher":"Wiley","issue":"15","license":[{"start":{"date-parts":[[2022,3,24]],"date-time":"2022-03-24T00:00:00Z","timestamp":1648080000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004085","name":"Ministry of Education, Science and Technology","doi-asserted-by":"publisher","award":["NRF\u20102021R1A2B5B01001793"],"award-info":[{"award-number":["NRF\u20102021R1A2B5B01001793"]}],"id":[{"id":"10.13039\/501100004085","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004085","name":"Ministry of Education, Science and Technology","doi-asserted-by":"publisher","award":["NRF\u20102020R1A2C200912712"],"award-info":[{"award-number":["NRF\u20102020R1A2C200912712"]}],"id":[{"id":"10.13039\/501100004085","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004085","name":"Ministry of Education, Science and Technology","doi-asserted-by":"publisher","award":["NRF\u20102019R1C1C1005403"],"award-info":[{"award-number":["NRF\u20102019R1C1C1005403"]}],"id":[{"id":"10.13039\/501100004085","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003625","name":"Ministry of Health and Welfare","doi-asserted-by":"publisher","award":["21B0601L1\u201001"],"award-info":[{"award-number":["21B0601L1\u201001"]}],"id":[{"id":"10.13039\/501100003625","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002701","name":"Ministry of Education","doi-asserted-by":"publisher","award":["NRF\u20102019R1C1C1005403"],"award-info":[{"award-number":["NRF\u20102019R1C1C1005403"]}],"id":[{"id":"10.13039\/501100002701","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"published-print":{"date-parts":[[2022,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Kidney organoids derived from human pluripotent stem cells (hPSCs) have extensive potential for disease modelling and regenerative medicine. However, the limited vascularization and immaturity of kidney organoids have been still remained to overcome. Extracellular matrix (ECM) can provide mechanical support and a biochemical microenvironment for cell growth and differentiation. Here in vitro methods using a kidney decellularized extracellular matrix (dECM) hydrogel to culture hPSC\u2010derived kidney organoids, which have extensive vascular network and their own endothelial cells, are reported. Single\u2010cell transcriptomics reveal that the vascularized kidney organoids cultured using the kidney dECM have more mature patterns of glomerular development and higher similarity to human kidney than those cultured without the kidney dECM. Differentiation of\n                    <jats:italic>\u03b1<\/jats:italic>\n                    \u2010galactosidase A (GLA)\u2010knock\u2010out hPSCs generated using CRISPR\/Cas9 into kidney organoids by the culture method using kidney dECM efficiently recapitulate Fabry nephropathy with vasculopathy. Transplantation of kidney organoids with kidney dECM into kidney of mouse accelerates the recruitment of endothelial cells from the host mouse kidney and maintains vascular integrity with the more organized slit diaphragm\u2010like structures than those without kidney dECM. The kidney dECM methodology for inducing extensive vascularization and maturation of kidney organoids can be applied to studies for kidney development, disease modeling, and regenerative medicine.\n                  <\/jats:p>","DOI":"10.1002\/advs.202103526","type":"journal-article","created":{"date-parts":[[2022,3,24]],"date-time":"2022-03-24T19:42:27Z","timestamp":1648150947000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":160,"title":["Kidney Decellularized Extracellular Matrix Enhanced the Vascularization and Maturation of Human Kidney Organoids"],"prefix":"10.1002","volume":"9","author":[{"given":"Jin Won","family":"Kim","sequence":"first","affiliation":[{"name":"Cell Death Disease Research Center College of Medicine The Catholic University of Korea  Seoul 06591 Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sun Ah","family":"Nam","sequence":"additional","affiliation":[{"name":"Cell Death 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