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Sperm motility can be impaired by both genetic and environmental factors, with asthenozoospermia being a common clinical presentation. Moreover, in the setting of assisted reproductive technology clinics, there is a distinct absence of effective and noninvasive technology to increase sperm motility without detriment to the sperm cells. Here, a new method is presented to boost sperm motility by increasing the intracellular rate of metabolic activity using high frequency ultrasound. An increase of 34% in curvilinear velocity (VCL), 10% in linearity, and 32% in the number of motile sperm cells is shown by rendering immotile sperm motile, after just 20\u00a0s exposure. A similar effect with an increase of 15% in VCL treating human sperm with the same setting is also identified. This cell level mechanotherapy approach causes no significant change in cell viability or DNA fragmentation index, and, as such, has the potential to be applied to encourage natural fertilization or less invasive treatment choices such as in vitro fertilization rather than intracytoplasmic injection.<\/jats:p>","DOI":"10.1002\/advs.202104362","type":"journal-article","created":{"date-parts":[[2022,2,9]],"date-time":"2022-02-09T07:47:20Z","timestamp":1644392840000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["High\u2010Frequency Ultrasound Boosts Bull and Human Sperm Motility"],"prefix":"10.1002","volume":"9","author":[{"given":"Junyang","family":"Gai","sequence":"first","affiliation":[{"name":"Department of Mechanical and Aerospace Engineering Monash University  Clayton Victoria 3800 Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Esma","family":"Dervisevic","sequence":"additional","affiliation":[{"name":"Department of Mechanical and Aerospace Engineering Monash University  Clayton Victoria 3800 Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Citsabehsan","family":"Devendran","sequence":"additional","affiliation":[{"name":"Department of Mechanical and Aerospace Engineering Monash University  Clayton Victoria 3800 Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Victor J.","family":"Cadarso","sequence":"additional","affiliation":[{"name":"Department of Mechanical and Aerospace Engineering Monash University  Clayton Victoria 3800 Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Moira K.","family":"O'Bryan","sequence":"additional","affiliation":[{"name":"Department of Mechanical and Aerospace Engineering Monash University  Clayton Victoria 3800 Australia"},{"name":"School of BioSciences Faculty of Science the University of Melbourne  Parkville Victoria 3010 Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reza","family":"Nosrati","sequence":"additional","affiliation":[{"name":"Department of Mechanical and Aerospace Engineering Monash University  Clayton Victoria 3800 Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7571-2526","authenticated-orcid":false,"given":"Adrian","family":"Neild","sequence":"additional","affiliation":[{"name":"Department of Mechanical and Aerospace Engineering Monash University  Clayton Victoria 3800 Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,2,9]]},"reference":[{"key":"e_1_2_10_1_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12958-015-0032-1"},{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.4161\/spmg.28218"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/1477-7827-12-2"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmr018"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-fluid-121108-145442"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-23773-x"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrurol.2017.175"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms9703"},{"key":"e_1_2_10_9_1","first-page":"1","author":"Madewell Z. 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