{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T15:35:50Z","timestamp":1790091350366,"version":"4.0.1"},"reference-count":57,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2022,3,27]],"date-time":"2022-03-27T00:00:00Z","timestamp":1648339200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2020A151501612"],"award-info":[{"award-number":["2020A151501612"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"published-print":{"date-parts":[[2022,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The clustered regularly interspaced short palindromic repeats (CRISPR) molecular system has emerged as a promising technology for the detection of nucleic acids. Herein, the development of a surface plasmon resonance (SPR) sensor that is functionalized with a layer of locally grown graphdiyne film, achieving excellent sensing performance when coupled with catalytically deactivated CRISPR\u2010associated protein 9 (dCas9), is reported. dCas9 protein is immobilized on the sensor surface and complexed with a specific single\u2010guide RNA, enabling the amplification\u2010free detection of target sequences within genomic DNA. The sensor, termed CRISPR\u2010SPR\u2010Chip, is used to successfully analyze recombinant plasmids with only three\u2010base mutations with a limit of detection as low as 1.3 fM. Real\u2010time monitoring CRISPR\u2010SPR\u2010Chip is used to analyze clinical samples of patients with Duchenne muscular dystrophy with two exon deletions, which are detected without any pre\u2010amplification step, yielding significantly positive results within 5\u00a0min. The ability of this novel CRISPR\u2010empowered SPR (CRISPR\u2010eSPR) sensing platform to rapidly, precisely, sensitively, and specifically detect a target gene sequence provides a new on\u2010chip optic approach for clinical gene analysis.<\/jats:p>","DOI":"10.1002\/advs.202105231","type":"journal-article","created":{"date-parts":[[2022,3,27]],"date-time":"2022-03-27T23:25:08Z","timestamp":1648423508000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":286,"title":["A Highly Sensitive CRISPR\u2010Empowered Surface Plasmon Resonance Sensor for Diagnosis of Inherited Diseases with Femtomolar\u2010Level Real\u2010Time Quantification"],"prefix":"10.1002","volume":"9","author":[{"given":"Fei","family":"Zheng","sequence":"first","affiliation":[{"name":"Shenzhen Engineering Laboratory of phosphorene and Optoelectronics International Collaborative Laboratory of 2D Materials for Optoelectronics Science and Technology of Ministry of Education Shenzhen Institute of Translational Medicine Department of Otolaryngology Shenzhen Second People's Hospital the First Affiliated Hospital Institute of Microscale Optoelectronics Shenzhen University  Shenzhen 518060 P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7624-3143","authenticated-orcid":false,"given":"Zhi","family":"Chen","sequence":"additional","affiliation":[{"name":"Shenzhen Engineering Laboratory of phosphorene and Optoelectronics International Collaborative Laboratory of 2D Materials for Optoelectronics Science and Technology of Ministry of Education Shenzhen Institute of Translational Medicine Department of Otolaryngology Shenzhen Second People's Hospital the First Affiliated Hospital Institute of Microscale Optoelectronics Shenzhen University  Shenzhen 518060 P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingfeng","family":"Li","sequence":"additional","affiliation":[{"name":"Shenzhen Engineering Laboratory of phosphorene and Optoelectronics International Collaborative Laboratory of 2D Materials for Optoelectronics Science and Technology of Ministry of Education Shenzhen Institute of Translational Medicine Department of Otolaryngology Shenzhen Second People's Hospital the First Affiliated Hospital Institute of Microscale Optoelectronics Shenzhen University  Shenzhen 518060 P.R. China"},{"name":"Shenzhen International Institute for Biomedical Research  Shenzhen 518110 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Wu","sequence":"additional","affiliation":[{"name":"Laboratory of Robotics and System Harbin Institute of Technology  Harbin 150001 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shenzhen Engineering Laboratory of phosphorene and Optoelectronics International Collaborative Laboratory of 2D Materials for Optoelectronics Science and Technology of Ministry of Education Shenzhen Institute of Translational Medicine Department of Otolaryngology Shenzhen Second People's Hospital the First Affiliated Hospital Institute of Microscale Optoelectronics Shenzhen University  Shenzhen 518060 P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guohui","family":"Nie","sequence":"additional","affiliation":[{"name":"Shenzhen Engineering Laboratory of phosphorene and Optoelectronics International Collaborative Laboratory of 2D Materials for Optoelectronics Science and Technology of Ministry of Education Shenzhen Institute of Translational Medicine Department of Otolaryngology Shenzhen Second People's Hospital the First Affiliated Hospital Institute of Microscale Optoelectronics Shenzhen University  Shenzhen 518060 P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongjian","family":"Xie","sequence":"additional","affiliation":[{"name":"Institute of Pediatrics Shenzhen Children's Hospital  Shenzhen Guangdong 518038 P. R. China"},{"name":"Shenzhen International Institute for Biomedical Research  Shenzhen Guangdong 518116 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0166-1973","authenticated-orcid":false,"given":"Han","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shenzhen Engineering Laboratory of phosphorene and Optoelectronics International Collaborative Laboratory of 2D Materials for Optoelectronics Science and Technology of Ministry of Education Shenzhen Institute of Translational Medicine Department of Otolaryngology Shenzhen Second People's Hospital the First Affiliated Hospital Institute of Microscale Optoelectronics Shenzhen University  Shenzhen 518060 P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,3,27]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2019.02.032"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-019-0214-5"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1910138"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2019.06.009"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2021.02.007"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-07324-5"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2020.112437"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201712217"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-017-0620-3"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2017.10.015"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.04.025"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.08.021"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-018-0049-z"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/d41586-020-03476-x"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41596-019-0210-2"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41421-018-0028-z"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aar6245"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/acssynbio.9b00209"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2017.04.016"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41551-019-0371-x"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2020.112316"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.0c00022"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.0c08165"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abc7802"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1039\/D0SC03084H"},{"key":"e_1_2_9_26_1","volume":"14","author":"Kim H.","year":"2020","journal-title":"ACS Nano"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2016.12.019"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.21769\/BioProtoc.3519"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2018.11.021"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2019.03.013"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abb2507"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aab2051"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-07882-8"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.3390\/nano9020165"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00339-018-1804-1"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijleo.2019.163430"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201600354"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.201601570"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201504407"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1039\/C6CC01856D"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2832-5"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41582-019-0203-3"},{"key":"e_1_2_9_43_1","first-page":"4757","volume":"123","author":"Julie Y. H.","year":"2018","journal-title":"Lancet Neurol."},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1136\/jmedgenet-2015-103387"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201803101"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac502561e"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1039\/c2nr12026g"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbon.2004.03.005"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.5b04057"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1103\/PhysRevLett.97.187401"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.3481"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2016.01.051"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.0c02439"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201605308"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature13769"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201902575"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2020.112363"}],"container-title":["Advanced Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.202105231","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/advs.202105231","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.202105231","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T22:51:20Z","timestamp":1759791080000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/advs.202105231"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,3,27]]},"references-count":57,"journal-issue":{"issue":"14","published-print":{"date-parts":[[2022,5]]}},"alternative-id":["10.1002\/advs.202105231"],"URL":"https:\/\/doi.org\/10.1002\/advs.202105231","archive":["Portico"],"relation":{},"ISSN":["2198-3844","2198-3844"],"issn-type":[{"value":"2198-3844","type":"print"},{"value":"2198-3844","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,3,27]]},"assertion":[{"value":"2021-11-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-03-27","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2105231"}}