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Its transmission capacity is approximately twice that of the original strain, with a shorter incubation period and higher viral load during infection. Importantly, the breakthrough infections of the Delta variant have continued to emerge in the first\u2010generation vaccine recipients. There is thus an urgent need to develop a novel vaccine with SARS\u2010CoV\u20102 variants as the major target. Here, receptor binding domain (RBD)\u2010conjugated nanoparticle vaccines targeting the Delta variant, as well as the early and Beta\/Gamma strains, are developed. Under both a single\u2010dose and a prime\u2010boost strategy, these RBD\u2010conjugated nanoparticle vaccines induce the abundant neutralizing antibodies (NAbs) and significantly protect hACE2 mice from infection by the authentic SARS\u2010CoV\u20102 Delta strain, as well as the early and Beta strains. Furthermore, the elicitation of the robust production of broader cross\u2010protective NAbs against almost all the notable SARS\u2010CoV\u20102 variants including the Omicron variant in rhesus macaques by the third re\u2010boost with trivalent vaccines is found. These results suggest that RBD\u2010based monovalent or multivalent nanoparticle vaccines provide a promising second\u2010generation vaccine strategy for SARS\u2010CoV\u20102 variants.<\/jats:p>","DOI":"10.1002\/advs.202105378","type":"journal-article","created":{"date-parts":[[2022,2,10]],"date-time":"2022-02-10T07:15:50Z","timestamp":1644477350000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Development of Receptor Binding Domain (RBD)\u2010Conjugated Nanoparticle Vaccines with Broad Neutralization against SARS\u2010CoV\u20102 Delta and Other Variants"],"prefix":"10.1002","volume":"9","author":[{"given":"Ran","family":"Chen","sequence":"first","affiliation":[{"name":"Institute of Human Virology Key Laboratory of Tropical Disease Control of Ministry Education Guangdong Engineering Research Center for Antimicrobial Agent and Immunotechnology Zhongshan School of Medicine Sun Yat\u2010sen University  Guangzhou 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