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The strategy of hydrogel\u2010based TEC holds considerable promise in circumventing these problems. Herein, a non\u2010toxic, biodegradable, and mechanically optimized double\u2010network (DN) hydrogel consisting of polyethylene glycol (PEG) and kartogenin (KGN)\u2010conjugated chitosan (CHI) is constructed using a simple soaking strategy. This PEG\u2010CHI\u2010KGN DN hydrogel possesses favorable architectures, suitable mechanics, remarkable cellular affinity, and sustained KGN release, which can facilitate the cartilage\u2010specific genes expression and extracellular matrix secretion of peripheral blood\u2010derived mesenchymal stem cells (PB\u2010MSCs). Notably, after tracing the transplanted cells by detecting the\u00a0rabbit sex\u2010determining region Y\u2010linked gene sequence, the allogeneic PB\u2010MSCs are found to survive\u00a0for even 3 months in the regenerated cartilage. Here, the long\u2010term release of KGN is able to efficiently and persistently activate multiple genes and signaling pathways to promote the chondrogenesis, chondrocyte differentiation, and survival of PB\u2010MSCs. Thus, the regenerated tissues exhibit well\u2010matched histomorphology and biomechanical performance such as native cartilage. Consequently, it is believed this innovative work can expand the choice for developing the next generation of orthopedic implants in the loadbearing region of a living body.<\/jats:p>","DOI":"10.1002\/advs.202105571","type":"journal-article","created":{"date-parts":[[2022,10,17]],"date-time":"2022-10-17T21:15:41Z","timestamp":1666041341000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":53,"title":["Kartogenin\u2010Conjugated Double\u2010Network Hydrogel Combined with Stem Cell Transplantation and Tracing for Cartilage Repair"],"prefix":"10.1002","volume":"9","author":[{"given":"You\u2010Rong","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Sports Medicine Beijing Key Laboratory of Sports Injuries Peking University Third Hospital  Beijing 100191 China"},{"name":"Institute of Sports Medicine Peking University  Beijing 100191 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