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To date, functional trachea regeneration with alternant cartilage\u2010fibrous tissue\u2010mimetic structure similar to that of the native trachea relying on the three\u2010dimensional (3D)\u00a0bioprinting technology has seen very limited breakthrough. This fact is mostly due to the lack of tissue\u2010specific bioinks suitable for both cartilage and vascularized fibrous tissue regeneration, as well as the need for firm interfacial integration between stiff and soft tissues. Here, a novel strategy is developed for 3D bioprinting of cartilage\u2010vascularized fibrous tissue\u2010integrated trachea (<jats:bold>CVFIT<\/jats:bold>), utilizing photocrosslinkable tissue\u2010specific bioinks. Both cartilage\u2010 and fibrous tissue\u2010specific bioinks created by this study provide suitable printability, favorable biocompatibility, and biomimetic microenvironments for chondrogenesis and vascularized fibrogenesis based on the multicomponent synergistic effect through the hybrid photoinitiated polymerization reaction. As such, the tubular analogs are successfully bioprinted and the ring\u2010to\u2010ring alternant structure is tightly integrated by the enhancement of interfacial bonding through the amidation reaction. The results from both the trachea regeneration and the in situ trachea reconstruction demonstrate the satisfactory tissue\u2010specific regeneration along with realization of mechanical and physiological functions. This study thus illustrates the 3D\u2010bioprinted native tissue\u2010like trachea as a promising alternative for clinical trachea reconstruction.<\/jats:p>","DOI":"10.1002\/advs.202202181","type":"journal-article","created":{"date-parts":[[2022,7,26]],"date-time":"2022-07-26T22:14:25Z","timestamp":1658873665000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":64,"title":["Functional Trachea Reconstruction Using 3D\u2010Bioprinted Native\u2010Like Tissue Architecture Based on Designable Tissue\u2010Specific Bioinks"],"prefix":"10.1002","volume":"9","author":[{"given":"Yingying","family":"Huo","sequence":"first","affiliation":[{"name":"Department of Plastic and Reconstructive Surgery Shanghai 9th People's Hospital Shanghai Jiao Tong University School of Medicine Shanghai Key Laboratory of Tissue Engineering  Shanghai 200011 P. R. China"},{"name":"National Tissue Engineering Center of China  Shanghai 200241 P. R. 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