{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T21:26:57Z","timestamp":1773955617556,"version":"3.50.1"},"reference-count":43,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2023,1,5]],"date-time":"2023-01-05T00:00:00Z","timestamp":1672876800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R35GM147019"],"award-info":[{"award-number":["R35GM147019"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008975","name":"University of Massachusetts Amherst","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008975","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Inflammasome activation is associated with a myriad of inflammatory diseases. However, existing methods provides a limited understanding of spatiotemporal kinetics of inflammasome activation, with restricted scope for early detection of associated treatment efficacy. This limitation offers an opportunity for the development of biocompatible in\u2010vivo inflammasome monitoring tools with translational prospects. To achieve this, they report developing a pair of lipid\u2010based nanoparticle systems, a reporter nanoparticle consisting of a caspase\u20101 activatable probe alone, and a theranostic nanoparticle combining the probe with an inflammasome\u2010inhibiting drug. This biocompatible platform enhances the probe's residence time in circulation by preventing its opsonization and allowing its sustained release over time. Their results demonstrate the specificity of reporter nanoparticles towards caspase\u20101 activity and provides early\u2010on monitoring of inflammasome activation both in\u2010vitro as well as in\u2010vivo. Additionally, the delivery of disulfiram, an inflammasome\u2010inhibiting drug, along with reporter probe using theranostic nanoparticles enables real\u2010time tracking of treatment efficacy in the gouty\u2010arthritis inflammatory model. In summary, they report an unparalleled pair of the inflammasome\u2010associated reporter and theranostic platforms suited not only for diagnostic applications but can also detect inflammasome\u2010targeted treatment efficiency in real\u2010time. These findings establish two novel, sensitive nanotools for non\u2010invasive evaluation of inflammasome\u2010targeted immunotherapy.<\/jats:p>","DOI":"10.1002\/advs.202204900","type":"journal-article","created":{"date-parts":[[2023,1,5]],"date-time":"2023-01-05T15:46:41Z","timestamp":1672933601000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Nanoreporter for Real\u2010Time Monitoring of Inflammasome Activity and Targeted Therapy"],"prefix":"10.1002","volume":"10","author":[{"given":"Dipika","family":"Nandi","sequence":"first","affiliation":[{"name":"Department of Chemical Engineering University of Massachusetts  Amherst MA 01003 USA"},{"name":"Department of Veterinary and Animal Sciences University of Massachusetts  Amherst MA 01003 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"III","given":"James","family":"Forster","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering University of Massachusetts  Amherst MA 01003 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anujan","family":"Ramesh","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering University of Massachusetts  Amherst MA 01003 USA"},{"name":"Department of Biomedical Engineering University of Massachusetts  Amherst MA 01003 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anh","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering University of Massachusetts  Amherst MA 01003 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hariharan","family":"Bharadwaj","sequence":"additional","affiliation":[{"name":"Department of Pathology UMass Chan Medical School\u2010Baystate  Springfield MA 01107 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5713-2202","authenticated-orcid":false,"given":"Ashish","family":"Kulkarni","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering University of Massachusetts  Amherst MA 01003 USA"},{"name":"Department of Veterinary and Animal Sciences University of Massachusetts  Amherst MA 01003 USA"},{"name":"Department of Biomedical Engineering University of Massachusetts  Amherst MA 01003 USA"},{"name":"Center for Bioactive Delivery Institute for Applied Life Sciences University of Massachusetts  Amherst MA 01003 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,1,5]]},"reference":[{"key":"e_1_2_7_1_1","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/4063562"},{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-021-00779-x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20172222"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.207365"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2070"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.01.063"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2021.167251"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.4016"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2019.119543"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep17205"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2016.06.011"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201703103"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2016.06.011"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3199-14.2015"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2327"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-3566-6_6"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2017.03.004"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202000648"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.7150\/ntno.62730"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202004172"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201901633"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.1c14082"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms2608"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfw011"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2009.03.034"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.00103"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M900528-MCP200"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41590-020-0669-6"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/clt2.12045"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2020.03.007"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1800562115"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.10972"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.2166"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201904364"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.2217\/17435889.1.3.297"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.3390\/nano7110355"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-7837-3_4"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21124294"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1021\/acscentsci.8b00218"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1080\/08958378.2016.1257664"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13075-018-1550-y"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.40650"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1801097"}],"container-title":["Advanced Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.202204900","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/advs.202204900","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.202204900","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T10:49:13Z","timestamp":1759747753000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/advs.202204900"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,1,5]]},"references-count":43,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2023,2]]}},"alternative-id":["10.1002\/advs.202204900"],"URL":"https:\/\/doi.org\/10.1002\/advs.202204900","archive":["Portico"],"relation":{},"ISSN":["2198-3844","2198-3844"],"issn-type":[{"value":"2198-3844","type":"print"},{"value":"2198-3844","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,1,5]]},"assertion":[{"value":"2022-08-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-01-05","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2204900"}}