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However, the oral fungal microbiota in COVID\u201019 remains unclear. This article aims to characterize the oral mycobiome in COVID\u201019 and recovered patients. Tongue coating specimens of 71 COVID\u201019 patients, 36 suspected cases (SCs), 22 recovered COVID\u201019 patients, 36 SCs who recovered, and 132 controls from Henan are collected and analyzed using internal transcribed spacer sequencing. The richness of oral fungi is increased in COVID\u201019 versus controls, and beta diversity analysis reveals separate fungal communities for COVID\u201019 and control. The ratio of <jats:italic>Ascomycota<\/jats:italic> and <jats:italic>Basidiomycota<\/jats:italic> is higher in COVID\u201019, and the opportunistic pathogens, including the genera <jats:italic>Candida<\/jats:italic>, <jats:italic>Saccharomyces<\/jats:italic>, and <jats:italic>Simplicillium<\/jats:italic>, are increased in COVID\u201019. The classifier based on two fungal biomarkers is constructed and can distinguish COVID\u201019 patients from controls in the training, testing, and independent cohorts. Importantly, the classifier successfully diagnoses SCs with positive specific severe acute respiratory syndrome coronavirus 2 immunoglobulin G antibodies as COVID\u201019 patients. The correlation between distinct fungi and bacteria in COVID\u201019 and control groups is depicted. These data suggest that the oral mycobiome may play a role in COVID\u201019.<\/jats:p>","DOI":"10.1002\/advs.202205058","type":"journal-article","created":{"date-parts":[[2023,4,29]],"date-time":"2023-04-29T09:00:38Z","timestamp":1682758838000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Oral Fungal Alterations in Patients with COVID\u201019 and Recovered Patients"],"prefix":"10.1002","volume":"10","author":[{"given":"Xiaobo","family":"Hu","sequence":"first","affiliation":[{"name":"Department of Infectious Diseases the First Affiliated Hospital of Zhengzhou University  #1 Jianshe East Road Zhengzhou 450052 P. R. China"},{"name":"Gene Hospital of Henan Province Precision Medicine Center the First Affiliated Hospital of Zhengzhou University  Zhengzhou 450052 P. R. 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