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For example, infections by human papillomavirus (HPV) or <jats:italic>Helicobacter pylori (H. pylori)<\/jats:italic> are the main cause of cervical and stomach cancers, respectively. COVID\u201019 and many cancers are treatable with early diagnoses using POCT. A variety of nucleic acid testing have been developed for use in resource\u2010limited environments. However, questions like unintegrated nucleic acid extraction, open detection systems increase the risk of cross\u2010contamination, and dependence on expensive equipment and alternating current (AC) power supply, significantly limit the application of POCT, especially for on\u2010site testing. In this paper, a simple portable platform is reported capable of rapid sample\u2010to\u2010answer testing within 30 min based on recombinase polymerase amplification (RPA) at a lower temperature, to detect SARS\u2010CoV\u20102 virus and <jats:italic>H. pylori<\/jats:italic> bacteria with a limit of detection as low as 4 \u00d7 10<jats:sup>2<\/jats:sup> copies mL<jats:sup>\u22121<\/jats:sup>. The platform used a battery\u2010powered portable reader for on\u2010chip one\u2010pot amplification and fluorescence detection, and can test for multiple (up to four) infectious pathogens simultaneously. This platform can provide an alternative method for fast and reliable on\u2010site diagnostic testing.<\/jats:p>","DOI":"10.1002\/advs.202205217","type":"journal-article","created":{"date-parts":[[2023,2,16]],"date-time":"2023-02-16T22:19:31Z","timestamp":1676585971000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":45,"title":["Portable Paper\u2010Based Nucleic Acid Enrichment for Field Testing"],"prefix":"10.1002","volume":"10","author":[{"given":"Junyang","family":"Mei","sequence":"first","affiliation":[{"name":"State Key Laboratory of Oncogenes and Related Genes Shanghai Cancer Institute Renji Hospital School of Medicine Shanghai Jiao Tong University  Shanghai 200032 China"},{"name":"Central Laboratory Renji Hospital School of Medicine Shanghai Jiao Tong University  Shanghai 200127 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