{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T00:22:21Z","timestamp":1776298941069,"version":"3.50.1"},"reference-count":44,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2023,2,25]],"date-time":"2023-02-25T00:00:00Z","timestamp":1677283200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,2,25]],"date-time":"2023-02-25T00:00:00Z","timestamp":1677283200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100011620","name":"Center for Life Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100011620","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32170470"],"award-info":[{"award-number":["32170470"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32270446"],"award-info":[{"award-number":["32270446"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"published-print":{"date-parts":[[2023,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Toxoplasma gondii (T. gondii)<\/jats:italic>\n                    is the causative agent of toxoplasmosis and can infect numerous warm\u2010blooded animals. An improved understanding of the fine structure of this parasite can help elucidate its replication mechanism. Previous studies have resolved the ultrastructure of the cytoskeleton using purified samples, which eliminates their cellular context. Here the application of cryo\u2010electron tomography to visualize\n                    <jats:italic>T. gondii<\/jats:italic>\n                    tachyzoites in their native state is reported. The fine structure and cellular distribution of the cytoskeleton are resolved and analyzed at nanometer resolution. Additionally, the tachyzoite structural characteristics are annotated during its endodyogeny for the first time. By comparing the structural features in mature tachyzoites and their daughter buds, it is proposed that the conoid fiber of the Apicomplexa originates from microtubules. This work represents the detailed molecular anatomy of\n                    <jats:italic>T. gondii<\/jats:italic>\n                    , particularly during the budding replication stage of tachyzoite, and provides a reference for further studies of this fascinating organism.\n                  <\/jats:p>","DOI":"10.1002\/advs.202206595","type":"journal-article","created":{"date-parts":[[2023,2,25]],"date-time":"2023-02-25T08:20:21Z","timestamp":1677313221000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Cryo\u2010Electron Tomography of\n                    <i>Toxoplasma gondii<\/i>\n                    Indicates That the Conoid Fiber May Be Derived from Microtubules"],"prefix":"10.1002","volume":"10","author":[{"given":"Zhixun","family":"Li","sequence":"first","affiliation":[{"name":"State Key Laboratory of Protein and Plant Gene Research, Peking\u2010Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, School of Life Sciences Peking University  Beijing 100871 P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenjing","family":"Du","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Protein and Plant Gene Research, Peking\u2010Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, School of Life Sciences Peking University  Beijing 100871 P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiong","family":"Yang","sequence":"additional","affiliation":[{"name":"MOE Key Laboratory of Gene Function and Regulation, State Key Laboratory of Biocontrol, School of Life Sciences Sun Yat\u2010Sen University  Guangzhou 510275 P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"De\u2010Hua","family":"Lai","sequence":"additional","affiliation":[{"name":"MOE Key Laboratory of Gene Function and Regulation, State Key Laboratory of Biocontrol, School of Life Sciences Sun Yat\u2010Sen University  Guangzhou 510275 P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhao\u2010Rong","family":"Lun","sequence":"additional","affiliation":[{"name":"MOE Key Laboratory of Gene Function and Regulation, State Key Laboratory of Biocontrol, School of Life Sciences Sun Yat\u2010Sen University  Guangzhou 510275 P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3520-5439","authenticated-orcid":false,"given":"Qiang","family":"Guo","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Protein and Plant Gene Research, Peking\u2010Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, School of Life Sciences Peking University  Beijing 100871 P. R. China"},{"name":"Changping Laboratory  Yard 28, Science Park Road Beijing 102206 P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,2,25]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1201\/9781420092370"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0090203"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182012000765"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-020-04445-z"},{"key":"e_1_2_8_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2003.12.009"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.2307\/3276925"},{"key":"e_1_2_8_5_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.112.16.2631"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(01)00289-4"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.1987.tb03162.x"},{"key":"e_1_2_8_7_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.115.5.1017"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.1996.tb04491.x"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-25309-9"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E03-07-0530"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-23351-1"},{"key":"e_1_2_8_11_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2111661119"},{"key":"e_1_2_8_12_1","first-page":"90","volume":"23","author":"Van Den Zypen E.","year":"1968","journal-title":"Bol. Chil. Parasitol."},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.123364"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02094-15"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2021.167187"},{"key":"e_1_2_8_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-019-0591-8"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004074"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.56635"},{"key":"e_1_2_8_18_1","doi-asserted-by":"crossref","unstructured":"L.Gui W. J.O'Shaughnessy K.Cai E.Reetz M. L.Reese D.Nicastro bioRxiv: 2022.04.23.4892872022 https:\/\/doi.org\/10.1101\/2022.04.23.489287.","DOI":"10.1101\/2022.04.23.489287"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2009.01399.x"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0955-0674(89)80029-8"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0169(1998)40:1<38::AID-CM4>3.0.CO;2-B"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.65.1.193"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200112086"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2017.131"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41596-020-0320-x"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsb.2005.07.007"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsb.2016.06.007"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4193"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1006\/jsbi.1996.0013"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-019-0580-y"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsb.2014.02.015"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-012387582-2\/50040-X"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/pro.3235"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.59.2.267"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsb.2011.12.017"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.20084"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsb.2012.09.006"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-020-01054-7"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-22108-0"}],"container-title":["Advanced Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.202206595","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/advs.202206595","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.202206595","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T19:09:30Z","timestamp":1771355370000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/advs.202206595"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,2,25]]},"references-count":44,"journal-issue":{"issue":"14","published-print":{"date-parts":[[2023,5]]}},"alternative-id":["10.1002\/advs.202206595"],"URL":"https:\/\/doi.org\/10.1002\/advs.202206595","archive":["Portico"],"relation":{},"ISSN":["2198-3844","2198-3844"],"issn-type":[{"value":"2198-3844","type":"print"},{"value":"2198-3844","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,2,25]]},"assertion":[{"value":"2022-11-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-02-25","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2206595"}}