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The aim is to identify circulating tumor cell (CTC)\u2010biomarkers and to identify an effective combination of TIC\u2010specific, repurposed federal drug administration (FDA)\u2010approved drugs. Three different types of high\u2010throughput screens targeting the TIC population are employed: these include a CD133 (+) cell viability screen, a NANOG expression screen, and a drug combination screen. When combined in a refined secondary screening approach that targets Nanog expression with the same FDA\u2010approved drug library, histone deacetylase (HDAC) inhibitor(s) combined with all\u2010trans retinoic acid (ATRA) demonstrate the highest efficacy for inhibition of TIC growth in vitro and in vivo. Addition of immune checkpoint inhibitor further decreases recurrence and extends PDX mouse survival. RNA\u2010seq analysis of TICs reveals that combined drug treatment reduces many Toll\u2010like receptors (TLR) and stemness genes through repression of the lncRNA\n                    <jats:italic>MIR22HG<\/jats:italic>\n                    . This downregulation induces PTEN and TET2, leading to loss of the self\u2010renewal property of TICs. Thus, CTC biomarker analysis would predict the prognosis and therapy response to this drug combination. In general, biomarker\u2010guided stratification of HCC patients and TIC\u2010targeted therapy should eradicate TICs to extend HCC patient survival.\n                  <\/jats:p>","DOI":"10.1002\/advs.202206812","type":"journal-article","created":{"date-parts":[[2023,3,23]],"date-time":"2023-03-23T00:07:13Z","timestamp":1679530033000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["RETRACTED: Profiling of Circulating Tumor Cells for Screening of Selective Inhibitors of Tumor\u2010Initiating Stem\u2010Like Cells"],"prefix":"10.1002","volume":"10","author":[{"given":"Chia\u2010Lin","family":"Chen","sequence":"first","affiliation":[{"name":"Departments of Molecular Microbiology and Immunology University of Southern California  Los Angeles CA 90033 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan Carlos","family":"Hernandez","sequence":"additional","affiliation":[{"name":"Departments of 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