{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T18:03:58Z","timestamp":1793556238076,"version":"4.5.0"},"reference-count":60,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2023,4,21]],"date-time":"2023-04-21T00:00:00Z","timestamp":1682035200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31890781"],"award-info":[{"award-number":["31890781"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81871204"],"award-info":[{"award-number":["81871204"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81901535"],"award-info":[{"award-number":["81901535"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004826","name":"Natural Science Foundation of Beijing Municipality","doi-asserted-by":"publisher","award":["7232203"],"award-info":[{"award-number":["7232203"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021YFC2700200"],"award-info":[{"award-number":["2021YFC2700200"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFC2702600"],"award-info":[{"award-number":["2022YFC2702600"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"published-print":{"date-parts":[[2023,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Nonobstructive azoospermia (NOA) is one of the most important causes of male infertility, accounting for 10\u201315% of infertile men worldwide. Among these, more than 70% of cases are idiopathic NOA (iNOA), whose pathogenesis and molecular basis remain unknown. This work profiles 3696 human testicular single\u2010cell transcriptomes from 17 iNOA patients, which are classified into four classes with different arrest periods and variable cell proportions based on the gene expression patterns and pathological features. Genes related to the cell cycle, energy production, and gamete generation show obvious abnormalities in iNOA germ cells. This work identifies several candidate causal genes for iNOA, including\n                    <jats:italic>CD164<\/jats:italic>\n                    ,\n                    <jats:italic>LELP1<\/jats:italic>\n                    , and\n                    <jats:italic>TEX38<\/jats:italic>\n                    , which are significantly downregulated in iNOA germ cells. Notably,\n                    <jats:italic>CD164<\/jats:italic>\n                    knockdown promotes apoptosis in spermatogonia. Cellular communications between spermatogonial stem cells and Sertoli cells are disturbed in iNOA patients. Moreover,\n                    <jats:italic>BOD1L2<\/jats:italic>\n                    ,\n                    <jats:italic>C1orf194<\/jats:italic>\n                    , and\n                    <jats:italic>KRTCAP2<\/jats:italic>\n                    are found to indicate testicular spermatogenic capacity in a variety of testicular diseases, such as Y\u2010chromosome microdeletions and Klinefelter syndrome. In general, this study analyzes the pathogenesis of iNOA from the perspective of germ cell development, transcription factor (TF) regulatory networks, as well as germ cell and somatic cell interactions, which provides new ideas for clinical diagnosis.\n                  <\/jats:p>","DOI":"10.1002\/advs.202206852","type":"journal-article","created":{"date-parts":[[2023,4,21]],"date-time":"2023-04-21T07:51:47Z","timestamp":1682063507000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["Deciphering the Molecular Characteristics of Human Idiopathic Nonobstructive Azoospermia from the Perspective of Germ Cells"],"prefix":"10.1002","volume":"10","author":[{"given":"Yidong","family":"Chen","sequence":"first","affiliation":[{"name":"Center for Reproductive Medicine Department of Obstetrics and Gynecology Peking University Third Hospital  Beijing 100191 China"},{"name":"National Clinical Research Center for Obstetrics and Gynecology  Beijing 100191 China"},{"name":"Key Laboratory of Assisted Reproduction (Peking University) Ministry of Education  Beijing 100191 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xixi","family":"Liu","sequence":"additional","affiliation":[{"name":"Center for Reproductive Medicine Department of Obstetrics and Gynecology Peking University Third Hospital  Beijing 100191 China"},{"name":"National Clinical Research Center for Obstetrics and Gynecology  Beijing 100191 China"},{"name":"Key Laboratory of Assisted Reproduction (Peking University) Ministry of Education  Beijing 100191 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Zhang","sequence":"additional","affiliation":[{"name":"Center for Reproductive Medicine Department of Obstetrics and Gynecology Peking University Third Hospital  Beijing 100191 China"},{"name":"National Clinical Research Center for Obstetrics and Gynecology  Beijing 100191 China"},{"name":"Key Laboratory of Assisted Reproduction (Peking University) Ministry of Education  Beijing 100191 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feiyin","family":"Zhu","sequence":"additional","affiliation":[{"name":"Center for Reproductive Medicine Department of Obstetrics and Gynecology Peking University Third Hospital  Beijing 100191 China"},{"name":"National Clinical Research Center for Obstetrics and Gynecology  Beijing 100191 China"},{"name":"Key Laboratory of Assisted Reproduction (Peking University) Ministry of Education  Beijing 100191 China"},{"name":"Peking\u2010Tsinghua Center for Life Sciences Peking University  Beijing 100871 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liying","family":"Yan","sequence":"additional","affiliation":[{"name":"Center for Reproductive Medicine Department of Obstetrics and Gynecology Peking University Third Hospital  Beijing 100191 China"},{"name":"National Clinical Research Center for Obstetrics and Gynecology  Beijing 100191 China"},{"name":"Key Laboratory of Assisted Reproduction (Peking University) Ministry of Education  Beijing 100191 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenhao","family":"Tang","sequence":"additional","affiliation":[{"name":"Center for Reproductive Medicine Department of Obstetrics and Gynecology Peking University Third Hospital  Beijing 100191 China"},{"name":"National Clinical Research Center for Obstetrics and Gynecology  Beijing 100191 China"},{"name":"Key Laboratory of Assisted Reproduction (Peking University) Ministry of Education  Beijing 100191 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhe","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Urology Peking University Third Hospital  Beijing 100191 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiang","family":"Liu","sequence":"additional","affiliation":[{"name":"Center for Reproductive Medicine Department of Obstetrics and Gynecology Peking University Third Hospital  Beijing 100191 China"},{"name":"National Clinical Research Center for Obstetrics and Gynecology  Beijing 100191 China"},{"name":"Key Laboratory of Assisted Reproduction (Peking University) Ministry of Education  Beijing 100191 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Urology Peking University Third Hospital  Beijing 100191 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2126-1376","authenticated-orcid":false,"given":"Jie","family":"Qiao","sequence":"additional","affiliation":[{"name":"Center for Reproductive Medicine Department of Obstetrics and Gynecology Peking University Third Hospital  Beijing 100191 China"},{"name":"National Clinical Research Center for Obstetrics and Gynecology  Beijing 100191 China"},{"name":"Key Laboratory of 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