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However, the detailed molecular mechanism remains elusive. Here, using unbiased protein affinity purification and RNA sequencing, it is found that TopBP1 is associated with pre\u2010ribosomal RNA (pre\u2010rRNA). Pre\u2010rRNA co\u2010localized with TopBP1 at DNA double\u2010strand breaks (DSBs). Similar to pre\u2010rRNA, ribosomal proteins also colocalize with TopBP1 at DSBs. The recruitment of TopBP1 to DSBs is suppressed when cells are transiently treated with RNA polymerase I inhibitor (Pol I\u2010i) to suppress pre\u2010rRNA biogenesis but not protein translation. Moreover, the BRCT4\u20105 of TopBP1 recognizes pre\u2010rRNA and forms liquid\u2013liquid phase separation (LLPS) with pre\u2010rRNA, which may be the molecular basis of DSB\u2010induced foci of TopBP1. Finally, Pol I\u2010i treatment impairs TopBP1\u2010associated cell cycle checkpoint activation and homologous recombination repair. Collectively, this study reveals that pre\u2010rRNA plays a key role in the TopBP1\u2010dependent DNA damage response.<\/jats:p>","DOI":"10.1002\/advs.202206931","type":"journal-article","created":{"date-parts":[[2023,8,19]],"date-time":"2023-08-19T00:36:09Z","timestamp":1692405369000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Pre\u2010rRNA Facilitates TopBP1\u2010Mediated DNA Double\u2010Strand Break Response"],"prefix":"10.1002","volume":"10","author":[{"given":"Di","family":"Xin","sequence":"first","affiliation":[{"name":"School of Life Sciences Westlake University  Hangzhou Zhejiang 310024 China"},{"name":"Department of Hepatobiliary and Pancreatic Surgery and Zhejiang Provincial Key Laboratory of Pancreatic Disease The First Affiliated Hospital Zhejiang University School of Medicine  Hangzhou Zhejiang 310003 China"},{"name":"Westlake Laboratory of Life Sciences and Biomedicine  Hangzhou Zhejiang 310024 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