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Inactivated whole virusor large protein fragments\u2010based severe acute respiratory syndrome coronavirus (SARS\u2010CoV\u20102) vaccines have high unnecessary antigenic load to induce allergenicity and\/orreactogenicity, which can be avoided by peptide vaccines of short peptide fragments that may induce highly targeted immune response. However, epitope identification and peptide delivery remain the major obstacles in developing peptide vaccines. Here, a multi\u2010source data integrated linear B\u2010cell epitope screening strategy is presented and a linear B\u2010cell epitope enriched hotspot region is identified in Spike protein, from which a monomeric peptide vaccine (Epitope25) is developed and applied to subcutaneously immunize wildtype BALB\/c mice. Indirect ELISA assay reveals specific and dose\u2010dependent binding between Epitope25 and serum IgG antibodies from immunized mice. The neutralizing activity of sera from vaccinated mice is validated by pseudo and live SARS\u2010CoV\u20102 wild\u2010type strain neutralization assays. Then a dissolvable microneedle array (DMNA) is developed to pain\u2010freely deliver Epitope25. Compared with intramuscular injection, DMNA and subcutaneous injection elicit neutralizing activities against SARS\u2010CoV\u20102 wild\u2010type strain as demonstrated by live SARS\u2010CoV\u20102 virus neutralization assay. No obvious damages are found in major organs of immunized mice. This study may lay the foundation for developing linear B\u2010cell epitope\u2010based vaccines against SARS\u2010CoV\u20102.<\/jats:p>","DOI":"10.1002\/advs.202207474","type":"journal-article","created":{"date-parts":[[2023,5,11]],"date-time":"2023-05-11T00:09:24Z","timestamp":1683763764000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["A Newly Identified Spike Protein Targeted Linear B\u2010Cell Epitope Based Dissolvable Microneedle Array Successfully Eliciting Neutralizing Activities against SARS\u2010CoV\u20102 Wild\u2010Type Strain in Mice"],"prefix":"10.1002","volume":"10","author":[{"given":"Lin","family":"Li","sequence":"first","affiliation":[{"name":"Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education Beijing Advanced Innovation Centre for Biomedical Engineering School of Engineering Medicine and School of Biological Science and Medical Engineering Beihang University  Beijing 100083 P. 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