{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T21:05:07Z","timestamp":1791666307713,"version":"4.3.4"},"reference-count":37,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2023,2,7]],"date-time":"2023-02-07T00:00:00Z","timestamp":1675728000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Male infertility caused by genetic mutations is an important type of infertility. Currently, there is no reliable method in the clinic to address this medical need. The emergence of mRNA therapy provides a possible strategy for restoring mutant genes in the reproductive system. However, effective delivery of mRNA to spermatocytes remains a formidable challenge. Here a series of cholesterol\u2010amino\u2010phosphate (CAP) lipids are reported by integrating three bioactive moieties into a geometric structure, which is favorable for mRNA delivery. The results demonstrate that CAP\u2010derived lipid nanoparticles (CAP LNPs) can deliver RNA including traditional mRNA and self\u2010amplifying RNA (saRNA) encoding DNA Meiotic Recombinase 1 (Dmc1) protein in spermatocytes and treat male infertility caused by the Dmc1 gene mutation. Notably, the delivery efficiency of CAP LNPs is significantly higher than that of the MC3 and ALC\u20100315 LNPs, which is consistent with the design of CAP molecules. More importantly, a single injection of CAP LNPs\u2013saRNA can produce Dmc1 protein for an extended period, which restores the spermatogenesis in the Dmc1 gene knockout mouse model. Overall, this study proves the concept of LNPs for the delivery of mRNA to spermatocytes, which provides a unique method to probe male infertility caused by the genetic mutation.<\/jats:p>","DOI":"10.1002\/advs.202300188","type":"journal-article","created":{"date-parts":[[2023,2,7]],"date-time":"2023-02-07T03:42:05Z","timestamp":1675741325000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":57,"title":["Cholesterol\u2010Amino\u2010Phosphate (CAP) Derived Lipid Nanoparticles for Delivery of Self\u2010Amplifying RNA and Restoration of Spermatogenesis in Infertile Mice"],"prefix":"10.1002","volume":"10","author":[{"given":"Shi","family":"Du","sequence":"first","affiliation":[{"name":"Division of Pharmaceutics &amp; Pharmacology College of Pharmacy The Ohio State University  Columbus OH 43210 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenqing","family":"Li","sequence":"additional","affiliation":[{"name":"Division of Pharmaceutics &amp; Pharmacology College of Pharmacy The Ohio State University  Columbus OH 43210 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuebao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Division of Pharmaceutics &amp; Pharmacology College of Pharmacy The Ohio State University  Columbus OH 43210 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yonger","family":"Xue","sequence":"additional","affiliation":[{"name":"Division of Pharmaceutics &amp; Pharmacology College of Pharmacy The Ohio State University  Columbus OH 43210 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xucheng","family":"Hou","sequence":"additional","affiliation":[{"name":"Division of Pharmaceutics &amp; Pharmacology College of Pharmacy The Ohio State University  Columbus OH 43210 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingyue","family":"Yan","sequence":"additional","affiliation":[{"name":"Division of Pharmaceutics &amp; 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Davis Heart &amp; Lung Research Institute Department of Radiation Oncology Center for Cancer Engineering Center for Cancer Metabolism Pelotonia Institute for Immune\u2010Oncology The Ohio State University  Columbus OH 43210 USA"},{"name":"Icahn Genomics Institute Precision Immunology Institute Department of Oncological Sciences Tisch Cancer Institute, Friedman Brain Institute Icahn School of Medicine at Mount Sinai  New York NY 10029 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,2,7]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(20)32667-2"},{"key":"e_1_2_8_1_2","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmab030"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/jog.12765"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(03)14845-3"},{"key":"e_1_2_8_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1406192"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1613608"},{"key":"e_1_2_8_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/82539"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymthe.2019.02.012"},{"key":"e_1_2_8_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41565-021-00898-0"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2035389"},{"key":"e_1_2_8_6_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2034577"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms14630"},{"key":"e_1_2_8_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41565-019-0591-y"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abc2315"},{"key":"e_1_2_8_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41565-020-0669-6"},{"key":"e_1_2_8_8_3","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abc5911"},{"key":"e_1_2_8_8_4","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2020401118"},{"key":"e_1_2_8_8_5","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aba9772"},{"key":"e_1_2_8_8_6","doi-asserted-by":"publisher","DOI":"10.1002\/btm2.10161"},{"key":"e_1_2_8_8_7","doi-asserted-by":"publisher","DOI":"10.1039\/D0BM01609H"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)68939-8"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.1c00244"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(00)80069-6"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1039\/jr9450000660"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41565-019-0600-1"},{"key":"e_1_2_8_14_1","first-page":"1525","volume":"72","author":"Israelachvili J. 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