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Although collagen has been widely used as a cell culture scaffold for cancer cells, there have been limitations when attempting to fabricate a tough collagen gel with cells like a cancer stroma. Here, rapid gelation of a collagen solution within a few minutes by transition metal complexation is demonstrated. Type I collagen solution at neutral pH shows rapid gelation with a transparency of 81% and a high modulus of 1,781\u00a0kPa by mixing with K\n                    <jats:sub>2<\/jats:sub>\n                    PtCl\n                    <jats:sub>4<\/jats:sub>\n                    solution within 3\u00a0min. Other transition metal ions also show the same rapid gelation, but not basic metal ions. Interestingly, although type I to IV collagen molecules show rapid gelation, other extracellular matrices \u00a0do not exhibit this phenomenon. Live imaging of colon cancer organoids in 3D culture indicates a collective migration property with modulating high elastic modulus, suggesting activation for metastasis progress. This technology will be useful as a new class of 3D culture for cells and organoids due to its facility for deep\u2010live observation and mechanical stiffness adjustment.\n                  <\/jats:p>","DOI":"10.1002\/advs.202302637","type":"journal-article","created":{"date-parts":[[2023,9,12]],"date-time":"2023-09-12T01:15:03Z","timestamp":1694481303000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Ultra\u2010Rapid and Specific Gelation of Collagen Molecules for Transparent and Tough Gels by Transition Metal Complexation"],"prefix":"10.1002","volume":"10","author":[{"given":"Tomoyuki","family":"Suezawa","sequence":"first","affiliation":[{"name":"Division of Applied Chemistry, Graduate School of Engineering Osaka University  2\u20101 Yamadaoka Suita Osaka 565\u20130871 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