{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T07:21:52Z","timestamp":1790752912504,"version":"4.1.0"},"reference-count":74,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2023,12,19]],"date-time":"2023-12-19T00:00:00Z","timestamp":1702944000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFA1103103"],"award-info":[{"award-number":["2022YFA1103103"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFA0913300"],"award-info":[{"award-number":["2022YFA0913300"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2019YFA0110001"],"award-info":[{"award-number":["2019YFA0110001"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81970818"],"award-info":[{"award-number":["81970818"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31970819"],"award-info":[{"award-number":["31970819"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32270784"],"award-info":[{"award-number":["32270784"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82371075"],"award-info":[{"award-number":["82371075"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Choroidal atrophy is a common fundus pathological change closely related to the development of age\u2010related macular degeneration (AMD), retinitis pigmentosa, and pathological myopia. Studies suggest that choroidal endothelial cells (CECs) that form the choriocapillaris vessels are the first cells lost in choroidal atrophy. It is found that endothelial cells derived from human pluripotent stem cells (hPSC\u2010ECs) through the <jats:italic>MESP1+<\/jats:italic> mesodermal progenitor stage express CECs\u2010specific markers and can integrate into choriocapillaris. Single\u2010cell RNA\u2010seq (scRNA\u2010seq) studies show that hPSC\u2010ECs upregulate angiogenesis and immune\u2010modulatory and neural protective genes after interacting with ex vivo ischemic choroid. In a rat model of choroidal ischemia (CI), transplantation of hPSC\u2010ECs into the suprachoroidal space increases choroid thickness and vasculature density. Close\u2010up examination shows that engrafted hPSC\u2010ECs integrate with all layers of rat choroidal vessels and last 90 days. Remarkably, EC transplantation improves the visual function of CI rats. The work demonstrates that hPSC\u2010ECs can be used to repair choroidal ischemia in the animal model, which may lead to a new therapy to alleviate choroidal atrophy implicated in dry AMD, pathological myopia, and other ocular diseases.<\/jats:p>","DOI":"10.1002\/advs.202302940","type":"journal-article","created":{"date-parts":[[2023,12,20]],"date-time":"2023-12-20T02:58:30Z","timestamp":1703041110000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Human Pluripotent Stem Cells Derived Endothelial Cells Repair Choroidal Ischemia"],"prefix":"10.1002","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3107-9482","authenticated-orcid":false,"given":"Mengda","family":"Li","sequence":"first","affiliation":[{"name":"Eye Center Beijing Tsinghua Changgung Hospital  Beijing 102218 China"},{"name":"Institute for Precision Medicine Tsinghua University  Beijing 100084 China"},{"name":"School of Clinical Medicine Tsinghua University  Beijing 100084 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2421-3062","authenticated-orcid":false,"given":"Peiliang","family":"Wang","sequence":"additional","affiliation":[{"name":"SXMU\u2010Tsinghua Collaborative Innovation Center for Frontier Medicine School of Medicine Tsinghua University  Beijing 100084 China"},{"name":"State Key Laboratory for Complex, Severe, and Rare Diseases Tsinghua University  Beijing 100084 China"},{"name":"Center for Stem Cell Biology and Regenerative Medicine School of Medicine Tsinghua University  Beijing 100084 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5402-8437","authenticated-orcid":false,"given":"Si Tong","family":"Huo","sequence":"additional","affiliation":[{"name":"Eye Center Beijing Tsinghua Changgung Hospital  Beijing 102218 China"},{"name":"Institute for Precision Medicine Tsinghua University  Beijing 100084 China"},{"name":"School of Clinical Medicine Tsinghua University  Beijing 100084 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2545-9451","authenticated-orcid":false,"given":"Hui","family":"Qiu","sequence":"additional","affiliation":[{"name":"SXMU\u2010Tsinghua Collaborative Innovation Center for Frontier Medicine School of Medicine Tsinghua University  Beijing 100084 China"},{"name":"State Key Laboratory for Complex, Severe, and Rare Diseases Tsinghua University  Beijing 100084 China"},{"name":"Center for Stem Cell Biology and Regenerative Medicine School of Medicine Tsinghua University  Beijing 100084 China"},{"name":"School of Life Sciences Tsinghua University  Beijing 100084 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chendi","family":"Li","sequence":"additional","affiliation":[{"name":"Eye Center Beijing Tsinghua Changgung Hospital  Beijing 102218 China"},{"name":"Institute for Precision Medicine Tsinghua University  Beijing 100084 China"},{"name":"School of Clinical Medicine Tsinghua University  Beijing 100084 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siyong","family":"Lin","sequence":"additional","affiliation":[{"name":"Eye Center Beijing Tsinghua Changgung Hospital  Beijing 102218 China"},{"name":"Institute for Precision Medicine Tsinghua University  Beijing 100084 China"},{"name":"School of Clinical Medicine Tsinghua University  Beijing 100084 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Libin","family":"Guo","sequence":"additional","affiliation":[{"name":"Eye Center Beijing Tsinghua Changgung Hospital  Beijing 102218 China"},{"name":"Institute for Precision Medicine Tsinghua University  Beijing 100084 China"},{"name":"School of Clinical Medicine Tsinghua University  Beijing 100084 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yicong","family":"Ji","sequence":"additional","affiliation":[{"name":"Eye Center Beijing Tsinghua Changgung Hospital  Beijing 102218 China"},{"name":"Institute for Precision Medicine Tsinghua University  Beijing 100084 China"},{"name":"School of Clinical Medicine Tsinghua University  Beijing 100084 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6932-2946","authenticated-orcid":false,"given":"Yonglin","family":"Zhu","sequence":"additional","affiliation":[{"name":"Center for Stem Cell Biology and Regenerative Medicine School of Medicine Tsinghua University  Beijing 100084 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6170-435X","authenticated-orcid":false,"given":"Jinyang","family":"Liu","sequence":"additional","affiliation":[{"name":"SXMU\u2010Tsinghua Collaborative Innovation Center for Frontier Medicine School of Medicine Tsinghua University  Beijing 100084 China"},{"name":"State Key Laboratory for Complex, Severe, and Rare Diseases Tsinghua University  Beijing 100084 China"},{"name":"Center for Stem Cell Biology and Regenerative Medicine School of Medicine Tsinghua University  Beijing 100084 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4136-0857","authenticated-orcid":false,"given":"Jianying","family":"Guo","sequence":"additional","affiliation":[{"name":"Center for Reproductive Medicine Department of Obstetrics and Gynaecology Peking University Third Hospital  Beijing 100191 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1820-0548","authenticated-orcid":false,"given":"Jie","family":"Na","sequence":"additional","affiliation":[{"name":"SXMU\u2010Tsinghua Collaborative Innovation Center for Frontier Medicine School of Medicine Tsinghua University  Beijing 100084 China"},{"name":"State Key Laboratory for Complex, Severe, and Rare Diseases Tsinghua University  Beijing 100084 China"},{"name":"Center for Stem Cell Biology and Regenerative Medicine School of Medicine Tsinghua University  Beijing 100084 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2708-2547","authenticated-orcid":false,"given":"Yuntao","family":"Hu","sequence":"additional","affiliation":[{"name":"Eye Center Beijing Tsinghua Changgung Hospital  Beijing 102218 China"},{"name":"Institute for Precision Medicine Tsinghua University  Beijing 100084 China"},{"name":"School of Clinical Medicine Tsinghua University  Beijing 100084 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,12,19]]},"reference":[{"key":"e_1_2_10_1_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22073689"},{"key":"e_1_2_10_2_1","volume-title":"The Ocular Circulation","author":"JW K.","year":"2010"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.preteyeres.2017.12.001"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.preteyeres.2019.100803"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.preteyeres.2009.12.002"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-072160561-6.50014-9"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.preteyeres.2018.07.002"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.09-3639"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0039-6257(99)00086-7"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dadm.2019.08.005"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.3109\/09273948.2015.1087576"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.survophthal.2016.10.003"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41433-022-02270-5"},{"key":"e_1_2_10_14_1","doi-asserted-by":"crossref","first-page":"351","DOI":"10.18502\/jovr.v15i3.7454","volume":"15","author":"Sabbaghi H.","year":"2020","journal-title":"J. Ophthalm. Vis. Res."},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/5.5.705"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2008.12.019"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1177\/11206721211067333"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1097\/IAE.0000000000001557"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(18)31550-2"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2019.07.011"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2214-109X(13)70145-1"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/aos.12631"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajo.2011.08.039"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.18-26086"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajo.2008.12.010"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pdpdt.2019.08.015"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2018.05.013"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.4114"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.preteyeres.2007.02.002"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2021.120713"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-016-0455-4"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1369\/jhc.4A6514.2005"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.01.015"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mvr.2018.12.002"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20190730"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2012.11.003"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0603501103"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0030-1253447"},{"key":"e_1_2_10_39_1","first-page":"494","volume":"26","author":"Huang J.","year":"2020","journal-title":"Mol Vis."},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22179218"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms160715086"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2022.856261"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature09002"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph16040500"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri2470"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1100646"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/79815"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2004-12-4716"},{"key":"e_1_2_10_49_1","first-page":"333","volume":"22","author":"Iwata R.","year":"2020","journal-title":"Neuro\u2010Oncology"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1534-5807(03)00395-2"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature09675"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2011.06.009"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.00299"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2017.03.026"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrn.2017.36"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-021-02203-1"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0027385"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12015-017-9750-4"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.1111\/aos.14773"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.1136\/bjophthalmol-2013-303143"},{"key":"e_1_2_10_61_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abm5559"},{"key":"e_1_2_10_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2021.101971"},{"key":"e_1_2_10_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.preteyeres.2021.100997"},{"key":"e_1_2_10_64_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-16312-7"},{"key":"e_1_2_10_65_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stemcr.2022.01.001"},{"key":"e_1_2_10_66_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1609583"},{"key":"e_1_2_10_67_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41551-021-00764-3"},{"key":"e_1_2_10_68_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1608368"},{"key":"e_1_2_10_69_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.preteyeres.2021.100950"},{"key":"e_1_2_10_70_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stemcr.2019.01.013"},{"key":"e_1_2_10_71_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-018-0934-x"},{"key":"e_1_2_10_72_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkp1005"},{"key":"e_1_2_10_73_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2019"},{"key":"e_1_2_10_74_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrep.2021.100916"}],"container-title":["Advanced Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.202302940","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T13:07:07Z","timestamp":1759756027000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/advs.202302940"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,12,19]]},"references-count":74,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2024,3]]}},"alternative-id":["10.1002\/advs.202302940"],"URL":"https:\/\/doi.org\/10.1002\/advs.202302940","archive":["Portico"],"relation":{},"ISSN":["2198-3844","2198-3844"],"issn-type":[{"value":"2198-3844","type":"print"},{"value":"2198-3844","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,12,19]]},"assertion":[{"value":"2023-05-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-12-19","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2302940"}}