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An important step in developing biosensors involves identifying reliable sensing probes against specific genetic targets for CT and NG. Here, the authors have designed single\u2010stranded oligonucleotides (ssDNAs) targeting mutually conserved genetic regions of cryptic plasmid and chromosomal DNA of both CT and NG. The 5\u2032\u2010 and 3\u2032\u2010 ends of these ssDNAs are differentially functionalized with thiol groups and coupled with gold nanoparticles (AuNP) to develop absorbance\u2010based assay. The AuNPs agglomerate selectively in the presence of its target DNA sequence and demonstrate a change in their surface plasmon resonance. The optimized assay is then used to detect both CT and NG DNA extracted from 60 anonymized clinical samples with a clinical sensitivity of \u223c100%. The limit of detection of the assays are found to be 7 and 5 copies\/\u00b5L for CT and NG respectively. Furthermore, it can successfully detect the DNA levels of these two bacteria without the need for DNA extraction and via a lateral flow\u2010based platform. These assays thus hold the potential to be employed in clinics for rapid and efficient monitoring of sexually transmitted infections.\n                  <\/jats:p>","DOI":"10.1002\/advs.202304009","type":"journal-article","created":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T07:27:14Z","timestamp":1698046034000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Highly\u2010Specific Single\u2010Stranded Oligonucleotides and Functional Nanoprobes for Clinical Determination of Chlamydia Trachomatis and Neisseria Gonorrhoeae Infections"],"prefix":"10.1002","volume":"10","author":[{"given":"Ketan","family":"Dighe","sequence":"first","affiliation":[{"name":"Department of Pediatrics Centre of Blood Oxygen Transport &amp; Hemostasis University of Maryland Baltimore School of Medicine  Baltimore Maryland 21201 USA"},{"name":"Department of Chemical &amp; Biochemical Engineering University of Maryland Baltimore County  Baltimore County Maryland 21250 USA"},{"name":"Department of Biomedical Engineering The Pennsylvania State University  University Park PA 16802 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Parikshit","family":"Moitra","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Centre of Blood Oxygen Transport &amp; Hemostasis University of Maryland Baltimore School of Medicine  Baltimore Maryland 21201 USA"},{"name":"Department of Nuclear Engineering The Pennsylvania State University  University Park PA 16802 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nivetha","family":"Gunaseelan","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Centre of Blood Oxygen Transport &amp; Hemostasis University of Maryland Baltimore School of Medicine  Baltimore Maryland 21201 USA"},{"name":"Department of Chemical &amp; Biochemical Engineering University of Maryland Baltimore County  Baltimore County Maryland 21250 USA"},{"name":"Department of Biomedical Engineering The Pennsylvania State University  University Park PA 16802 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maha","family":"Alafeef","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Centre of Blood Oxygen Transport &amp; Hemostasis University of Maryland Baltimore School of Medicine  Baltimore Maryland 21201 USA"},{"name":"Department of Chemical &amp; Biochemical Engineering University of Maryland Baltimore County  Baltimore County Maryland 21250 USA"},{"name":"Department of Nuclear Engineering The Pennsylvania State University  University Park PA 16802 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tor","family":"Jensen","sequence":"additional","affiliation":[{"name":"Cancer Center at Illinois University of Illinois Urbana\u2010Champaign  405 N. Mathews Ave. Urbana IL 61801\u20102325 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carla","family":"Rafferty","sequence":"additional","affiliation":[{"name":"Department of Family Medicine Carle Health  1818 E Windsor Rd. 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