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The traditional method to study neuronal ion\u2010channel activities heavily relies on the whole\u2010cell patch clamp as the industry standard. However, this technique is both technically challenging and labour\u2010intensive, while involving the complexity of keeping cells alive with low throughput. Therefore, the shortcomings are limiting the efficiency of ion\u2010channel\u2010related neuroscience research and drug testing. Here, this work reports a new system of integrating neuron membranes with organic microelectrode arrays (OMEAs) for ion\u2010channel\u2010related drug studies. This work demonstrates that the supported lipid bilayers (SLBs) derived from both neuron\u2010like (neuroblastoma) cells and primary neurons are integrated with OMEAs for the first time. The increased expression of voltage\u2010gated calcium (CaV) ion channels on differentiated SH\u2010SY5Y SLBs \u00a0compared to non\u2010differentiated ones is sensed electrically. Also, dose\u2010response of the CaV ion\u2010channel blocking effect on primary cortical neuronal SLBs from rats is monitored. The dose range causing ion channel blocking is comparable to literature. This system overcomes the major challenges from traditional methods (e.g., patch clamp) and showcases an easy\u2010to\u2010test, rapid, ultra\u2010sensitive, cell\u2010free, and high\u2010throughput platform to monitor dose\u2010dependent ion\u2010channel blocking effects on native neuronal membranes.<\/jats:p>","DOI":"10.1002\/advs.202304301","type":"journal-article","created":{"date-parts":[[2023,12,1]],"date-time":"2023-12-01T19:03:19Z","timestamp":1701457399000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Microelectrode Arrays Measure Blocking of Voltage\u2010Gated Calcium Ion Channels on Supported Lipid Bilayers Derived from Primary Neurons"],"prefix":"10.1002","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3689-4283","authenticated-orcid":false,"given":"Zixuan","family":"Lu","sequence":"first","affiliation":[{"name":"Department of Chemical Engineering and Biotechnology University of Cambridge  Philippa Fawcett Drive Cambridge CB3 0AS UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8621-1260","authenticated-orcid":false,"given":"Chiara","family":"Barberio","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering and Biotechnology University of Cambridge  Philippa Fawcett Drive Cambridge CB3 0AS UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Fernandez\u2010Villegas","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering and Biotechnology University of Cambridge  Philippa Fawcett Drive Cambridge CB3 0AS UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aimee","family":"Withers","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering and Biotechnology University of Cambridge  Philippa Fawcett Drive Cambridge CB3 0AS UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"Wheeler","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering and Biotechnology University of Cambridge  Philippa Fawcett Drive Cambridge CB3 0AS UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5480-4483","authenticated-orcid":false,"given":"Konstantinos","family":"Kallitsis","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering and Biotechnology University of Cambridge  Philippa Fawcett Drive Cambridge CB3 0AS UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eleonora","family":"Martinelli","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering and Biotechnology University of Cambridge  Philippa Fawcett Drive Cambridge CB3 0AS UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0197-0290","authenticated-orcid":false,"given":"Achilleas","family":"Savva","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering and Biotechnology University of Cambridge  Philippa Fawcett Drive Cambridge CB3 0AS UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Becky M.","family":"Hess","sequence":"additional","affiliation":[{"name":"Pacific Northwest National Laboratory  902 Battelle Boulevard Richland WA 99\u00a0354 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna\u2010Maria","family":"Pappa","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering and Biotechnology University of Cambridge  Philippa Fawcett Drive Cambridge CB3 0AS UK"},{"name":"Department of Biomedical Engineering Khalifa University of Science and Technology  Abu Dhabi 127788 UAE"},{"name":"Healthcare Engineering Innovation Center (HEIC) Khalifa University of Science and Technology  Abu Dhabi 127\u00a0788 UAE"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriele S. 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