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However, low cDC1 proportion, complex process, and high cost severely hindered cDC1 generation and application. Herein, the study proposes an in situ cDC1 recruitment and activation strategy with simultaneous inhibiting cancer stemness for inducing robust CTL responses and enhancing the anti\u2010tumor effect. Fms\u2010like tyrosine kinase 3 ligand (FLT3L), Poly I:C, and Nap\u2010CUM (NCUM), playing the role of cDC1 recruitment, cDC1 activation, inducing antigen release and decreasing tumor cell stemness, respectively, are co\u2010encapsulated in an in situ hydrogel vaccine (FP\/NCUM\u2010Gel). FP\/NCUM\u2010Gel is gelated in situ after intra\u2010tumoral injection. With the near\u2010infrared irradiation, tumor cell immunogenic cell death occurred, tumor antigens and immunogenic signals are released in situ. cDC1 is recruited to tumor tissue and activated for antigen cross\u2010presentation, followed by migrating to lymph nodes and activating CTLs. Furthermore, tumor cell stemness are inhibited by napabucasin, which can help CTLs to achieve comprehensive tumor killing. Collectively, the proposed strategy of cDC1 in situ recruitment and activation combined with stemness inhibition provides great immune response and anti\u2010tumor potential, providing new ideas for clinical tumor vaccine design.<\/jats:p>","DOI":"10.1002\/advs.202305832","type":"journal-article","created":{"date-parts":[[2024,4,2]],"date-time":"2024-04-02T16:40:01Z","timestamp":1712076001000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["In Situ Hydrogel Modulates\u00a0cDC1\u2010Based Antigen Presentation and Cancer Stemness to Enhance Cancer Vaccine Efficiency"],"prefix":"10.1002","volume":"11","author":[{"given":"Tong","family":"Gao","sequence":"first","affiliation":[{"name":"Department of Pharmaceutics Key Laboratory of Chemical Biology (Ministry of Education) NMPA Key Laboratory for Technology Research and Evaluation of Drug Products School of Pharmaceutical Sciences Cheeloo College of Medicine Shandong 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