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The investigation revealed that the absence of TSP50 compromised the integrity of the intestinal mucosal barrier in murine subjects. This disruption facilitated direct contact between intestinal bacteria and the intestinal epithelium, thereby increasing susceptibility to colitis. Mechanistic analysis indicated that TSP50 deficiency in intestinal stem cells (ISCs) triggered aberrant activation of the TGF\u2010\u03b2 signaling pathway and impeded the differentiation of goblet cells in mice, leading to impairment of mucosal permeability. By inhibiting the TGF\u2010\u03b2 pathway, the functionality of the intestinal mucosal barrier is successfully restored and mitigated colitis in TSP50\u2010deficient mice. In conclusion, TSP50 played a crucial role in maintaining the intestinal mucosal barrier function and exhibited the preventive effect against the development of colitis by regulating the TGF\u2010\u03b2 signaling pathway.<\/jats:p>","DOI":"10.1002\/advs.202305893","type":"journal-article","created":{"date-parts":[[2024,1,8]],"date-time":"2024-01-08T09:29:13Z","timestamp":1704706153000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["TSP50 Attenuates DSS\u2010Induced Colitis by Regulating TGF\u2010\u03b2 Signaling Mediated Maintenance of Intestinal Mucosal Barrier Integrity"],"prefix":"10.1002","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8229-1638","authenticated-orcid":false,"given":"Jiawei","family":"Li","sequence":"first","affiliation":[{"name":"NMPA Key Laboratory for Quality Control of Cell and Gene Therapy Medicine Products Northeast Normal University  Changchun 130024 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