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This work shows that the activation of specific oncogenes leads to elevated expression of mRNAs and their corresponding proteins in extracellular vesicles (EVs) circulating in blood. Utilizing an immune lipoplex nanoparticle (ILN) biochip assay, these findings demonstrate that glypican 1 (GPC1) mRNA expression in the exosomes\u2010rich (Exo) EV subpopulation and GPC1 membrane protein (mProtein) expression in the microvesicles\u2010rich (MV) EV subpopulation, particularly the tumor associated microvesicles (tMV), served as a viable biomarker for PDAC. A combined analysis effectively discriminated early\u2010stage PDAC patients from benign pancreatic diseases and healthy donors in sizable clinical from multiple hospitals. Furthermore, among late\u2010stage PDAC patients undergoing chemotherapy, lower GPC1 tMV\u2010mProtein and Exo\u2010mRNA expression before treatment correlated significantly with prolonged overall survival. These findings underscore the potential of vesicular GPC1 expression for early PDAC screenings and chemotherapy prognosis.<\/jats:p>","DOI":"10.1002\/advs.202306373","type":"journal-article","created":{"date-parts":[[2024,1,11]],"date-time":"2024-01-11T02:06:49Z","timestamp":1704938809000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":41,"title":["Extracellular Vesicular Analysis of Glypican 1 mRNA\u00a0and Protein for Pancreatic Cancer Diagnosis and Prognosis"],"prefix":"10.1002","volume":"11","author":[{"given":"Hong","family":"Li","sequence":"first","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering Ohio State University  Columbus OH 43210 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chi\u2010Ling","family":"Chiang","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering Ohio State University  Columbus OH 43210 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kwang Joo","family":"Kwak","sequence":"additional","affiliation":[{"name":"Spot Biosystems Ltd.  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