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However, several challenges need to be addressed before its widespread clinical application. These challenges include the low delivery efficiencies to target cells, the actual efficiency of the genome\u2010editing process, and the precision with which the CRISPR\u2010Cas system operates. Herein, the study presents a Magnetic Nanoparticle\u2010Assisted Genome Editing (<jats:bold>MAGE<\/jats:bold>) platform, which significantly improves the transfection efficiency, biocompatibility, and genome\u2010editing accuracy of CRISPR\u2010Cas9 technology. To demonstrate the feasibility of the developed technology, MAGE is applied to correct the mutated MeCP2 gene\u00a0in induced pluripotent stem cell\u2010derived neural progenitor cells (iPSC\u2010NPCs) from a Rett syndrome patient. By combining magnetofection and magnetic\u2010activated cell sorting, MAGE achieves higher multi\u2010plasmid delivery (99.3%) and repairing efficiencies (42.95%) with significantly shorter incubation times than conventional transfection agents without size limitations on\u00a0plasmids. The repaired iPSC\u2010NPCs showed similar characteristics as wild\u2010type neurons when they differentiated into neurons, further validating MAGE and its potential for future clinical applications. In short, the developed nanobio\u2010combined CRISPR\u2010Cas9 technology offers the potential for various clinical applications, particularly in stem cell therapies targeting different genetic diseases.<\/jats:p>","DOI":"10.1002\/advs.202306432","type":"journal-article","created":{"date-parts":[[2024,4,22]],"date-time":"2024-04-22T09:39:25Z","timestamp":1713778765000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Magnetic Nanoparticle\u2010Assisted Non\u2010Viral CRISPR\u2010Cas9 for Enhanced Genome Editing to Treat Rett Syndrome"],"prefix":"10.1002","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1897-1166","authenticated-orcid":false,"given":"Hyeon\u2010Yeol","family":"Cho","sequence":"first","affiliation":[{"name":"Department of Chemistry and Chemical Biology Rutgers, The State University of New Jersey  Piscataway NJ 08854 USA"},{"name":"Department of Chemical and Biomolecular Engineering Sogang University  Seoul 04107 South Korea"},{"name":"Department of Bio and Fermentation Convergence Technology Kookmin University  Seoul 02707 South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Myungsik","family":"Yoo","sequence":"additional","affiliation":[{"name":"W. 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