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Whether EAA regulates the activity of miR has never been demonstrated. Here, as proof\u2010of\u2010concept, a tryptophan (Trp, an EAA) complex containing Argonaute 2 (Ago2) and miRs including miR\u2010193a (Trp\/Ago2\/miR\u2010193a) is identified. Trp binds miR\u2010193a\u20103p and interacts with Ago2. Trp\/Ago2\/miR\u2010193a increases miR\u2010193a\u20103p activity via enhancing Argonaute 2 (Ago2) RNase activity. Other miRs including miR\u2010103 and miR\u2010107 in the Trp complex enhance miR\u2010193a activity by targeting the same genes. Mechanistically, the Trp\/Ago2\/miR\u2010193a complex interacts with Trp\u2010binding pockets of the PIWI domain of Ago2 to enhance Ago2 mediated miR activity. This newly formed Ago2\/Trp\/miR\u2010193a\u20103p complex is more efficient than miR\u2010193a\u20103p alone in inhibiting the expression of targeted genes and inhibiting colon cancer liver metastasis. 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