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However, it is challenging to develop hemostatic adhesives that are fast\u2010acting, strongly adherent, long\u2010lasting, and biocompatible for treating hemorrhage. In this study, a sequential crosslinking fibrin glue (SCFG) is developed, of which the first network of the fibrin glue forms in situ within 2\u00a0s to act as an initial physical barrier and locks the gelatin methacryloyl precursor for tight construction of the second network to enhance wet adhesion and durability for tissues covered with blood. The sequential crosslinking glue can provide large pressures (\u2248280\u00a0mmHg of burst pressure), makes strong (38\u00a0kPa of shear strength) and tough (\u224860\u00a0J\u00a0m\n                    <jats:sup>\u22122<\/jats:sup>\n                    of interfacial toughness) interfaces with wet tissues, and outperforms commercial hemostatic agents and gelatin methacryloyl. SCFG are demonstrated as an effective and safe sealant to enhance the treatment outcomes of bleeding tissues in rat, rabbit, and pig models. The ultrafast gelation, strong adhesion and durability, excellent compatibility, and easy manufacture of SCFG make it a promising hemostatic adhesive for clinical applications.\n                  <\/jats:p>","DOI":"10.1002\/advs.202308171","type":"journal-article","created":{"date-parts":[[2023,12,10]],"date-time":"2023-12-10T21:45:54Z","timestamp":1702244754000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":42,"title":["Sequential\u2010Crosslinking Fibrin Glue for Rapid and Reinforced Hemostasis"],"prefix":"10.1002","volume":"11","author":[{"given":"Lisha","family":"Yu","sequence":"first","affiliation":[{"name":"Department of Hepatobiliary and Pancreatic Surgery The Second Affiliated Hospital School of Medicine Zhejiang University  Hangzhou Zhejiang 310009 China"},{"name":"Key Laboratory of Precision Diagnosis and Treatment for Hepatobiliary and Pancreatic Tumor of Zhejiang Province  Hangzhou Zhejiang 310009 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Laboratory of Macromolecular Synthesis and Functionalization Department of Polymer Science and Engineering Zhejiang University  Hangzhou Zhejiang 310027 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weilin","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary and Pancreatic Surgery The Second Affiliated Hospital School of Medicine Zhejiang University  Hangzhou Zhejiang 310009 China"},{"name":"Key Laboratory of Precision Diagnosis and Treatment for Hepatobiliary and Pancreatic Tumor of Zhejiang Province  Hangzhou Zhejiang 310009 China"},{"name":"Research Center of Diagnosis and Treatment Technology for Hepatocellular Carcinoma of Zhejiang Province  Hangzhou Zhejiang 310009 China"},{"name":"National Innovation Center for Fundamental Research on Cancer Medicine  Hangzhou Zhejiang 310009 China"},{"name":"Cancer Center Zhejiang University  Hangzhou Zhejiang 310058 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