{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T15:05:14Z","timestamp":1781708714966,"version":"3.54.5"},"reference-count":33,"publisher":"Wiley","issue":"47","license":[{"start":{"date-parts":[[2024,10,28]],"date-time":"2024-10-28T00:00:00Z","timestamp":1730073600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,10,28]],"date-time":"2024-10-28T00:00:00Z","timestamp":1730073600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100018904","name":"Beijing Xisike Clinical Oncology Research Foundation","doi-asserted-by":"publisher","award":["Y\u2010HS202102\u20100152"],"award-info":[{"award-number":["Y\u2010HS202102\u20100152"]}],"id":[{"id":"10.13039\/100018904","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018806","name":"Department of Science and Technology of Hubei Province","doi-asserted-by":"publisher","award":["2022BCA021"],"award-info":[{"award-number":["2022BCA021"]}],"id":[{"id":"10.13039\/501100018806","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Immunotherapies employing PD\u20101\/PD\u2010L1 immune checkpoint inhibitors (ICIs) are vital for primary liver cancer (PLC), but response rates remain unsatisfying. Accurate differentiation of responders from non\u2010responders to immunotherapy is imperative. Here, single\u2010cell\u2010scaled mass cytometry analysis on sequential peripheral blood mononuclear cells (PBMCs) from ICI\u2010treated PLC patients is conducted, and tissue residence of immune subpopulations is assessed via multiplex immunohistochemistry. In the discovery cohort (n = 24), responders have lower baseline B cell and HLA\u2010DR\n                    <jats:sup>+<\/jats:sup>\n                    CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cell, and higher CD14\n                    <jats:sup>+<\/jats:sup>\n                    CD16\n                    <jats:sup>\u2212<\/jats:sup>\n                    classical monocyte (CM) proportions. CMs decrease more in responders PBMCs, while HLA\u2010DR\n                    <jats:sup>+<\/jats:sup>\n                    CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells conformably amplify after ICI\u2010exposure. Responsive individuals display upregulated exhaustion and activation markers in peripheral immune lineages. In the expanded cohort of 77 patients, the augment of the B cells in non\u2010responders is re\u2010confirmed. Responders demonstrate much higher enrichment of B cells or tertiary lymphoid structures in tumor compared to non\u2010responders. A prospective model that excelled in early discrimination of responders is developed using generalized linear model and achieves a satisfactory AUC over 0.9 in all three independent cohorts. Integratedly, the study unveils dynamic immune landscapes in PLC patients undergoing ICI\u2010based therapy, aiding in PLC patient stratification for ICI\u2010based treatment and fostering new response monitoring strategies.\n                  <\/jats:p>","DOI":"10.1002\/advs.202309631","type":"journal-article","created":{"date-parts":[[2024,10,28]],"date-time":"2024-10-28T14:47:24Z","timestamp":1730126844000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Deciphering the Multifaceted Immune Landscape of Unresectable Primary Liver Cancer to Predict Immunotherapy Response"],"prefix":"10.1002","volume":"11","author":[{"given":"Jun","family":"Xue","sequence":"first","affiliation":[{"name":"Cancer Center Union Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan 430022 China"},{"name":"Institute of Radiation Oncology Union Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan 430022 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuai","family":"Yang","sequence":"additional","affiliation":[{"name":"Fudan University Shanghai Cancer Center Department of Oncology Shanghai Medical College Fudan University  Shanghai 200032 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Si\u2010Si","family":"Zhang","sequence":"additional","affiliation":[{"name":"Cancer Center Union Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan 430022 China"},{"name":"Hubei Key Laboratory of Precision Radiation Oncology  Wuhan 430022 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Fan","sequence":"additional","affiliation":[{"name":"Cancer Center Union Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan 430022 China"},{"name":"Department of Pathology Union Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan 430022 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zi\u2010Long","family":"Wu","sequence":"additional","affiliation":[{"name":"Cancer Center Union Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan 430022 China"},{"name":"Hubei Key Laboratory of Precision Radiation Oncology  Wuhan 430022 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng\u2010Jun","family":"Sui","sequence":"additional","affiliation":[{"name":"The International Cooperation Laboratory on Signal Transduction Eastern Hepatobiliary Surgery Hospital Naval Medical University  Shanghai 200438 China"},{"name":"National Center for Liver Cancer  Shanghai 200441 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong\u2010Qiang","family":"Yang","sequence":"additional","affiliation":[{"name":"Cancer Center Union Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan 430022 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China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"De\u2010Jun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Cancer Center Union Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan 430022 China"},{"name":"Institute of Radiation Oncology Union Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan 430022 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin\u2010Yao","family":"Qiu","sequence":"additional","affiliation":[{"name":"Fudan University Shanghai Cancer Center Department of Oncology Shanghai Medical College Fudan University  Shanghai 200032 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Cancer Center Union Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan 430022 China"},{"name":"Institute of Radiation Oncology Union Hospital 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