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This is primarily due to its aggressiveness and intra\u2010tumoral heterogeneity, which limits complete surgical resection and reduces the efficacy of existing treatments. The existence of oncostreams\u2014neuropathological structures comprising aligned spindle\u2010like cells from both tumor and non\u2010tumor origins\u2010 is discovered earlier. Oncostreams are closely linked to glioma aggressiveness and facilitate the spread into adjacent healthy brain tissue. A unique molecular signature intrinsic to oncostreams, with overexpression of key genes (i.e., COL1A1, ACTA2) that drive the tumor's mesenchymal transition and malignancy is also identified. Pre\u2010clinical studies on genetically engineered mouse models demonstrated that COL1A1 inhibition disrupts oncostreams, modifies TME, reduces mesenchymal gene expression, and extends survival. An in vitro model using GFP+ NPA cells to investigate how various treatments affect oncostream dynamics is developed. Analysis showed that factors such as cell density, morphology, neurotransmitter agonists, calcium chelators, and cytoskeleton\u2010targeting drugs influence oncostream formation. This data illuminate the patterns of glioma migration and suggest anti\u2010invasion strategies that can improve GBM patient outcomes when combined with traditional therapies. This work highlights the potential of targeting oncostreams to control glioma invasion and enhance treatment efficacy.<\/jats:p>","DOI":"10.1002\/advs.202309796","type":"journal-article","created":{"date-parts":[[2024,2,22]],"date-time":"2024-02-22T03:07:01Z","timestamp":1708571221000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Spatiotemporal Insights into Glioma Oncostream Dynamics: Unraveling Formation, Stability, and Disassembly Pathways"],"prefix":"10.1002","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3968-7810","authenticated-orcid":false,"given":"Syed M.","family":"Faisal","sequence":"first","affiliation":[{"name":"Department of Neurosurgery University of Michigan Medical School  Ann Arbor Michigan 48108 USA"},{"name":"Department of Cell and Developmental Biology University of Michigan Medical School  Ann Arbor Michigan 48108 USA"},{"name":"Rogel Cancer Centre University of Michigan 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