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However, the challenge persists in protein activity preservation, significantly complicating the functional design of these coatings. Herein, an active dual\u2010protein surface engineering strategy assisted by a facile stepwise protein\u2013protein interactions assembly (SPPIA) method for catheters to reduce clot formation and infection is proposed. This strategy is realized first by the partial oxidation of bovine serum albumin (BSA) and lysozyme (LZM) for creating stable nucleation platforms via hydrophobic interaction, followed by the assembly of nonoxidized BSA (pI, the isoelectric point, \u22484.7) and LZM (pI \u224811) through electrostatic interaction owing to their opposite charge under neutral conditions. The SPPIA method effectively preserves the conformation and functionality of both BSA and LZM, thus endowing the resultant coating with potent antithrombotic and bactericidal properties. Furthermore, the stable nucleation platform ensures the adhesion and durability of the coating, resisting thrombosis and bacterial proliferation even after 15 days of PBS immersion. Overall, the SPPIA approach not only provides a new strategy for the fabrication of active protein coatings but also shows promise for the surface engineering technology of catheters.<\/jats:p>","DOI":"10.1002\/advs.202310259","type":"journal-article","created":{"date-parts":[[2024,3,1]],"date-time":"2024-03-01T00:49:40Z","timestamp":1709254180000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Active Dual\u2010Protein Coating Assisted by Stepwise Protein\u2013Protein Interactions Assembly Reduces Thrombosis and Infection"],"prefix":"10.1002","volume":"11","author":[{"given":"Wentai","family":"Zhang","sequence":"first","affiliation":[{"name":"Dongguan Key Laboratory of Smart Biomaterials and Regenerative Medicine The Tenth Affiliated Hospital Southern Medical University  Dongguan Guangdong 523000 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