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In this study, cartilage lesion\u2010localized hydrogel microspheres are developed to remodel the in situ efferocytosis to reverse cartilage senescence and recruit endogenous stem cells to accelerate cartilage repair. Specifically, aldehyde\u2010 and methacrylic anhydride (MA)\u2010modified hyaluronic acid hydrogel microspheres (AHM), loaded with pro\u2010apoptotic liposomes (liposomes encapsulating ABT263, A\u2010Lipo) and PDGF\u2010BB, namely A\u2010Lipo\/PAHM, are prepared by microfluidic and photo\u2010cross\u2010linking techniques. By a degraded porcine cartilage explant OA model, the in situ cartilage lesion location experiment illustrated that aldehyde\u2010functionalized microspheres promote affinity for degraded cartilage. In vitro data showed that A\u2010Lipo induced apoptosis of senescent chondrocytes (Sn\u2010chondrocytes), which can then be phagocytosed by the efferocytosis of macrophages, and remodeling efferocytosis facilitated the protection of normal chondrocytes and maintained the chondrogenic differentiation capacity of MSCs. In vivo experiments confirmed that hydrogel microspheres localized to cartilage lesion reversed cartilage senescence and promoted cartilage repair in OA. It is believed this in situ efferocytosis remodeling strategy can be of great significance for tissue regeneration in aging\u2010related diseases.<\/jats:p>","DOI":"10.1002\/advs.202400345","type":"journal-article","created":{"date-parts":[[2024,3,13]],"date-time":"2024-03-13T07:04:43Z","timestamp":1710313483000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":51,"title":["In Situ Remodeling of Efferocytosis via Lesion\u2010Localized Microspheres to Reverse Cartilage Senescence"],"prefix":"10.1002","volume":"11","author":[{"given":"Wei","family":"Xiong","sequence":"first","affiliation":[{"name":"Department of Foot and Ankle Surgery Beijing Tongren Hospital Capital Medical University  Beijing 100730 P. R. 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