{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T18:58:59Z","timestamp":1789153139432,"version":"build-2803163510"},"reference-count":36,"publisher":"Wiley","issue":"22","license":[{"start":{"date-parts":[[2024,3,29]],"date-time":"2024-03-29T00:00:00Z","timestamp":1711670400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82170506"],"award-info":[{"award-number":["82170506"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Aortic root aneurysm is a potentially life\u2010threatening condition that may lead to aortic rupture and is often associated with genetic syndromes, such as Marfan syndrome (MFS). Although studies with MFS animal models have provided valuable insights into the pathogenesis of aortic root aneurysms, this understanding of the transcriptomic and epigenomic landscape in human aortic root tissue remains incomplete. This knowledge gap has impeded the development of effective targeted therapies. Here, this study performs the first integrative analysis of single\u2010nucleus multiomic (gene expression and chromatin accessibility) and spatial transcriptomic sequencing data of human aortic root tissue under healthy and MFS conditions. Cell\u2010type\u2010specific transcriptomic and cis\u2010regulatory profiles in the human aortic root are identified. Regulatory and spatial dynamics during phenotypic modulation of vascular smooth muscle cells (VSMCs), the cardinal cell type, are delineated. Moreover, candidate key regulators driving the phenotypic modulation of VSMC, such as\n                    <jats:italic>FOXN3<\/jats:italic>\n                    ,\n                    <jats:italic>TEAD1<\/jats:italic>\n                    ,\n                    <jats:italic>BACH2<\/jats:italic>\n                    , and\n                    <jats:italic>BACH1<\/jats:italic>\n                    , are identified. In vitro experiments demonstrate that FOXN3 functions as a novel key regulator for maintaining the contractile phenotype of human aortic VSMCs through targeting\n                    <jats:italic>ACTA2<\/jats:italic>\n                    . These findings provide novel insights into the regulatory and spatial dynamics during phenotypic modulation in the aneurysmal aortic root of humans.\n                  <\/jats:p>","DOI":"10.1002\/advs.202400444","type":"journal-article","created":{"date-parts":[[2024,3,29]],"date-time":"2024-03-29T14:50:55Z","timestamp":1711723855000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Single\u2010Nucleus Multiomic Analyses Identifies Gene Regulatory Dynamics of Phenotypic Modulation in Human Aneurysmal Aortic Root"],"prefix":"10.1002","volume":"11","author":[{"given":"Xuanyu","family":"Liu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Cardiovascular Disease National Center for Cardiovascular Diseases Beijing Key Laboratory for Molecular Diagnostics of Cardiovascular Diseases Center of Laboratory Medicine Fuwai Hospital Chinese Academy of Medical Sciences and Peking Union Medical College  Beijing 100037 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingyi","family":"Zeng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Cardiovascular Disease National Center for Cardiovascular Diseases Beijing Key Laboratory for Molecular Diagnostics of Cardiovascular Diseases Center of Laboratory Medicine Fuwai Hospital Chinese Academy of Medical Sciences and Peking Union Medical College  Beijing 100037 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hang","family":"Yang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Cardiovascular Disease National Center for Cardiovascular Diseases Beijing Key Laboratory for Molecular Diagnostics of Cardiovascular Diseases Center of Laboratory Medicine Fuwai Hospital Chinese Academy of Medical Sciences and Peking Union Medical College  Beijing 100037 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenke","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Cardiovascular Disease National Center for Cardiovascular Diseases Beijing Key Laboratory for Molecular Diagnostics of Cardiovascular Diseases Center of Laboratory Medicine Fuwai Hospital Chinese Academy of Medical Sciences and Peking Union Medical College  Beijing 100037 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qianlong","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Cardiovascular Disease National Center for Cardiovascular Diseases Beijing Key Laboratory for Molecular Diagnostics of Cardiovascular Diseases Center of Laboratory Medicine Fuwai Hospital Chinese Academy of Medical Sciences and Peking Union Medical College  Beijing 100037 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kunlun","family":"Yin","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Cardiovascular Disease National Center for Cardiovascular Diseases Beijing Key Laboratory for Molecular Diagnostics of Cardiovascular Diseases Center of Laboratory Medicine Fuwai Hospital Chinese Academy of Medical Sciences and Peking Union Medical College  Beijing 100037 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zihang","family":"Pan","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pathophysiology School of Basic Medical Sciences State Key Laboratory of Vascular Homeostasis and Remodeling Peking University  Beijing 100191 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pathophysiology School of Basic Medical Sciences State Key Laboratory of Vascular Homeostasis and Remodeling Peking University  Beijing 100191 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingyao","family":"Luo","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Cardiovascular Disease National Center for Cardiovascular Diseases Beijing Key Laboratory for Molecular Diagnostics of Cardiovascular Diseases Center of Laboratory Medicine Fuwai Hospital Chinese Academy of Medical Sciences and Peking Union Medical College  Beijing 100037 China"},{"name":"Center of Vascular Surgery Fuwai Hospital Chinese Academy of Medical Sciences  Beijing 100037 China"},{"name":"Department of Vascular Surgery Fuwai Yunnan Cardiovascular Hospital Affiliated Cardiovascular Hospital of Kunming Medical University  Kunming Yunnan 650102 China"},{"name":"Department of Vascular Surgery Central\u2010China Subcenter of National Center for Cardiovascular Diseases Henan Cardiovascular Disease Center Fuwai Central\u2010China Cardiovascular Hospital Central China Fuwai Hospital of Zhengzhou University  Zhengzhou 450046 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chang","family":"Shu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Cardiovascular Disease National Center for Cardiovascular Diseases Beijing Key Laboratory for Molecular Diagnostics of Cardiovascular Diseases Center of Laboratory Medicine Fuwai Hospital Chinese Academy of Medical Sciences and Peking Union Medical College  Beijing 100037 China"},{"name":"Center of Vascular Surgery Fuwai Hospital Chinese Academy of Medical Sciences  Beijing 100037 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1060-0355","authenticated-orcid":false,"given":"Zhou","family":"Zhou","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Cardiovascular Disease National Center for Cardiovascular Diseases Beijing Key Laboratory for Molecular Diagnostics of Cardiovascular Diseases Center of Laboratory Medicine Fuwai Hospital Chinese Academy of Medical Sciences and Peking Union Medical College  Beijing 100037 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,3,29]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2019.12.043"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/HCO.0000000000000902"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-030-80614-9_8"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.5435\/JAAOS-D-16-00143"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41572-021-00298-7"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jvssci.2021.04.001"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.120.314670"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.120.046528"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.122.317953"},{"key":"e_1_2_9_10_1","first-page":"25","volume":"3","author":"Preissl 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