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However, the mechanism underlying their extended lifespan and significance in laryngeal squamous cell carcinoma (LSCC) remains unclear. Herein, it is observed that apoptosis of TANs is significantly delayed owing to induction by tumor\u2010derived G\u2010CSF and GM\u2010CSF through the activation of the PI3K\u2010AKT signaling pathway, upregulation of anti\u2010apoptotic Mcl\u20101 expression, and downregulation of activated Caspase\u20103 levels. It is found that prolonged survival of TANs leads to the accumulation of aged CXCR4<jats:sup>+<\/jats:sup> neutrophils that exhibit potent immunosuppressive properties and are associated with poor patient prognosis. Furthermore, extended survival promotes the enhanced immunosuppressive function of CD8<jats:sup>+<\/jats:sup> T cells by TANs, thereby facilitating the in vitro and in vivo progression and growth of human LSCC tumors. Importantly, this effect could be reversed by blocking G\u2010CSF and GM\u2010CSF stimulation of neutrophils. 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