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The native transposases <jats:italic>cgSpy<\/jats:italic> and <jats:italic>cvSpy<\/jats:italic> displayed transposition activities of approximately 85% and 35% compared to the hyperactive <jats:italic>piggyBac<\/jats:italic> transposase (<jats:italic>hyPB<\/jats:italic>). The cgSpy transposon showed unique characteristics, including a lack of overproduction inhibition and reduced efficiency for insertion sizes between 3.1 to 8.5\u00a0kb. Integration preferences of <jats:italic>cgSpy<\/jats:italic> are found in genes and regulatory regions, making it suitable for genetic manipulation. Evaluation in T\u2010cell engineering demonstrated that <jats:italic>cgSpy<\/jats:italic>\u2010mediated chimeric antigen receptor (CAR) modification is comparable to the PB system, indicating its potential utility in cell therapy. This study unveils the promising application of the active native transposase, <jats:italic>Spy<\/jats:italic>, from <jats:italic>Colletes gigas<\/jats:italic>, as a valuable tool for genetic engineering, particularly in T\u2010cell manipulation.<\/jats:p>","DOI":"10.1002\/advs.202400969","type":"journal-article","created":{"date-parts":[[2024,5,22]],"date-time":"2024-05-22T03:31:03Z","timestamp":1716348663000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["A Naturally Active <i>Spy<\/i> Transposon Discovered from the Insect Genome of <i>Colletes gigas<\/i> as a Promising Novel Gene Transfer Tool"],"prefix":"10.1002","volume":"11","author":[{"given":"Mohamed","family":"Diaby","sequence":"first","affiliation":[{"name":"College of Animal Science &amp; Technology Yangzhou University  Yangzhou Jiangsu 225009 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