{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T19:16:37Z","timestamp":1792350997646,"version":"4.4.0"},"reference-count":89,"publisher":"Wiley","issue":"39","license":[{"start":{"date-parts":[[2024,9,4]],"date-time":"2024-09-04T00:00:00Z","timestamp":1725408000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>N<jats:sup>6<\/jats:sup>\u2010methyladenosine (m<jats:sup>6<\/jats:sup>A) is the most prevalent internal modification of mRNA and plays an important role in regulating plant growth. However, there is still a lack of effective tools to precisely modify m<jats:sup>6<\/jats:sup>A sites of individual transcripts in plants. Here, programmable m<jats:sup>6<\/jats:sup>A editing tools are developed by combining CRISPR\/dCas13(Rx) with the methyltransferase <jats:italic>GhMTA<\/jats:italic> (<jats:bold>T<\/jats:bold>argeted RNA <jats:bold>M<\/jats:bold>ethylation <jats:bold>E<\/jats:bold>ditor, <jats:bold>TME<\/jats:bold>) or the demethyltransferase <jats:italic>GhALKBH10<\/jats:italic> (<jats:bold>T<\/jats:bold>argeted RNA <jats:bold>D<\/jats:bold>emethylation <jats:bold>E<\/jats:bold>ditor, <jats:bold>TDE<\/jats:bold>). These editors enable efficient deposition or removal of m<jats:sup>6<\/jats:sup>A modifications at targeted sites of endo\u2010transcripts <jats:italic>GhECA1<\/jats:italic> and <jats:italic>GhDi19<\/jats:italic> within a broad editing window ranging from 0 to 46\u00a0nt. TDE editor significantly decreases m<jats:sup>6<\/jats:sup>A levels by 24%\u201376%, while the TME editor increases m<jats:sup>6<\/jats:sup>A enrichment, ranging from 1.37\u2010 to 2.51\u2010fold. Furthermore, installation and removal of m<jats:sup>6<\/jats:sup>A modifications play opposing roles in regulating <jats:italic>GhECA1<\/jats:italic> and <jats:italic>GhDi19<\/jats:italic> mRNA transcripts, which may be attributed to the fact that their m<jats:sup>6<\/jats:sup>A sites are located in different regions of the genes. Most importantly, targeting the <jats:italic>GhDi19<\/jats:italic> transcript with TME editor plants results in a significant increase in root length and enhanced drought resistance. Collectively, these m<jats:sup>6<\/jats:sup>A editors can be applied to study the function of specific m<jats:sup>6<\/jats:sup>A modifications and have the potential for future applications in crop improvement.<\/jats:p>","DOI":"10.1002\/advs.202401118","type":"journal-article","created":{"date-parts":[[2024,9,4]],"date-time":"2024-09-04T07:20:59Z","timestamp":1725434459000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["CRISPR\/dCas13(Rx) Derived RNA N<sup>6<\/sup>\u2010methyladenosine (m<sup>6<\/sup>A) Dynamic Modification in Plant"],"prefix":"10.1002","volume":"11","author":[{"given":"Lu","family":"Yu","sequence":"first","affiliation":[{"name":"National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural University  Wuhan 430070 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muna","family":"Alariqi","sequence":"additional","affiliation":[{"name":"National Key Laboratory of 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