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Moreover, current therapies primarily targeting inflammation can lead to immunosuppression\u2010related complications. Human amniotic epithelial stem cells (hAESCs), which exhibit low immunogenicity and ethical acceptability, have gained attention as potential therapeutics. In this study, it is demonstrated that their encapsulation in a hydrogel and administration via anal injection enhanced the colonic mucosal barrier repair in a murine colitis model induced by dextran sodium sulfate during the recovery phase. The underlying mechanism involved the release of exosomes from hAESCs enriched with microRNA\u201023a\u20103p, which post\u2010transcriptionally reduced tumor necrosis factor receptor 1 expression, suppressing the nuclear factor\u2010\u03baB pathway in colonic epithelial cells, thus played a key role in inflammation. The novel approach shows potential for IBD treatment by restoring intestinal epithelial homeostasis without the immunosuppressive therapy\u2010associated risks. 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