{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,10]],"date-time":"2026-11-10T18:58:34Z","timestamp":1794337114872,"version":"4.9.0"},"reference-count":60,"publisher":"Wiley","issue":"30","license":[{"start":{"date-parts":[[2024,6,14]],"date-time":"2024-06-14T00:00:00Z","timestamp":1718323200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32070119"],"award-info":[{"award-number":["32070119"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The rise of antibiotic resistance poses a significant public health crisis, particularly due to limited antimicrobial options for the treatment of infections with Gram\u2010negative pathogens. Here, an antimicrobial peptide (AMP) SR25 is characterized, which effectively kills both Gram\u2010negative and Gram\u2010positive bacteria through a unique dual\u2010targeting mechanism without detectable resistance. Meanwhile, an SR25\u2010functionalized hydrogel is developed for the efficient treatment of infected diabetic wounds. SR25 is obtained through genome mining from an uncultured bovine enteric actinomycete named\n                    <jats:italic>Nonomuraea Jilinensis<\/jats:italic>\n                    sp. nov. Investigations reveal that SR25 has two independent cellular targets, disrupting bacterial membrane integrity and restraining the activity of succinate:quinone oxidoreductase (SQR). In a diabetic mice wound infection model, the SR25\u2010incorporated hydrogel exhibits high efficacy against mixed infections of\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    (\n                    <jats:italic>E. coli<\/jats:italic>\n                    ) and methicillin\u2010resistant\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    (MRSA), accelerating wound healing. Overall, these findings demonstrate the therapeutic potential of SR25 and highlight the value of mining drugs with multiple mechanisms from uncultured animal commensals for combating challenging bacterial pathogens.\n                  <\/jats:p>","DOI":"10.1002\/advs.202401793","type":"journal-article","created":{"date-parts":[[2024,6,14]],"date-time":"2024-06-14T09:36:00Z","timestamp":1718357760000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":49,"title":["Dual\u2010Mechanism Peptide SR25 has Broad Antimicrobial Activity and Potential Application for Healing Bacteria\u2010infected Diabetic Wounds"],"prefix":"10.1002","volume":"11","author":[{"given":"Xue\u2010Yue","family":"Luo","sequence":"first","affiliation":[{"name":"Department of Preventive Veterinary Medicine College of Veterinary Medicine Jilin University  Changchun Jilin 130062 P. R. 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