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In addition to transcriptional processes, RNA\u2010binding proteins (RBPs) are emerging as crucial post\u2010transcriptional players in regulating CM DACCA. However, whether post\u2010transcriptional regulation of CM DACCA by RBPs could effectively trigger daughter CM formation remains unknown. By performing integrated bioinformatic analysis of snRNA\u2010seq data from neonatal and adult hearts, this study identified Hnrnpa1 as a potential RBP regulating CM DACCA. Hnrnpa1 expression decreased significantly during postnatal heart development. With the use of \u03b1\u2010MHC\u2010H2B\u2010mCh\/CAG\u2010eGFP\u2010anillin transgenic mice, Hnrnpa1 overexpression promoted CM DACCA, thereby triggering daughter CM formation and enhancing cardiac repair after myocardial infarction (MI). In contrast, CRISPR\/Cas9 technology is used to generate CM\u2010specific Hnrnpa1 knockout mice. Hnrnpa1 knockout inhibited cardiac regeneration and worsened cardiac function in the neonatal MI model. Nanopore RNA sequencing, RIP assay, IP\u2010MS, MeRIP\u2010qPCR, PAR\u2010CLIP and luciferase reporter experiments showed that Hnrnpa1 induced Mettl3 post\u2010transcriptional splicing to inhibit m6A\u2010dependent Pbx1 and E2F1 degradation, thereby increasing Runx1, Ccne1, Cdk2 and Ccnb2 expression to promote CM DACCA. In conclusion, Hnrnpa1 triggered daughter CM formation by promoting CM DACCA in a post\u2010transcriptional manner, indicating that Hnrnpa1 might serve as a promising target in cardiac repair post\u2010MI.<\/jats:p>","DOI":"10.1002\/advs.202402371","type":"journal-article","created":{"date-parts":[[2024,11,19]],"date-time":"2024-11-19T04:40:10Z","timestamp":1731991210000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["RNA\u2010Binding Protein Hnrnpa1 Triggers Daughter Cardiomyocyte Formation by Promoting Cardiomyocyte Dedifferentiation and Cell Cycle Activity in a Post\u2010Transcriptional Manner"],"prefix":"10.1002","volume":"12","author":[{"given":"Chuling","family":"Li","sequence":"first","affiliation":[{"name":"Department of Cardiology State Key Laboratory of Organ Failure Research Nanfang Hospital Southern Medical University  Guangzhou 510515 China"},{"name":"Guangdong Provincial Key Laboratory of Cardiac 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