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Neutrophils have a short lifespan and cannot be mass\u2010cultured in vitro. Therefore, developing more stable artificial neutrophils (AN) in a controllable manner has become a research focus. However, existing AN lack chemotaxis, which is the ability to migrate toward high\u2010signal\u2010concentration positions in a dynamic blood\u2010 flow environment. Supplying AN with chemotaxis is key to designing AN that are more similar to natural neutrophils in terms of morphology and function. In this study, micrometer\u2010sized, spherical, biocompatible AN are developed. These AN consist of zeolitic imidazolate framework\u20108 nanoparticles encapsulating two enzymes, coacervate droplet frameworks, and outer phospholipid bilayers carrying enzymes. The AN exhibit responsiveness to elevated hydrogen peroxide levels at inflammation sites, actively chemotaxing toward these sites along concentration gradients. They also demonstrate effective combat against <jats:italic>Staphylococcus aureus<\/jats:italic> infections. The capabilities of the AN are further validated through in vitro experiments and in vivo evaluations using vascular graft infection models. This study replicates natural neutrophils in terms of chemical composition, functionality, and physiological impact. It introduces new ideas for advancing the development of advanced artificial cells.<\/jats:p>","DOI":"10.1002\/advs.202402768","type":"journal-article","created":{"date-parts":[[2024,6,14]],"date-time":"2024-06-14T09:01:02Z","timestamp":1718355662000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Artificial Neutrophils Against Vascular Graft Infection"],"prefix":"10.1002","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8274-389X","authenticated-orcid":false,"given":"Wentao","family":"Jiang","sequence":"first","affiliation":[{"name":"Department of Vascular Surgery Cardiovascular center Nanjing Drum Tower Hospital The Affiliated Hospital of Nanjing University Medical School  Nanjing 210008 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huizi","family":"Xu","sequence":"additional","affiliation":[{"name":"National and Local Joint Engineering Research Center of Biomedical 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