{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:23:52Z","timestamp":1786976632552,"version":"build-2736575974"},"reference-count":72,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,11,5]],"date-time":"2024-11-05T00:00:00Z","timestamp":1730764800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82271428\/ 82301346\/82130029"],"award-info":[{"award-number":["82271428\/ 82301346\/82130029"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    EED (embryonic ectoderm development) is a core subunit of the polycomb repressive complex 2 (PRC2), which senses the trimethylation of histone H3 lysine 27 (H3K27). However, its biological function in cerebellar development remains unknown. Here, we show that EED deletion from neural stem cells (NSCs) or cerebellar granule cell progenitors (GCPs) leads to reduced GCPs proliferation, cell death, cerebellar hypoplasia, and motor deficits in mice. Joint profiling of transcripts and ChIP\u2010seq analysis in cerebellar granule cells reveals that EED regulates bunches of genes involved in cerebellar development. EED ablation exhibits overactivation of a developmental repressor long non\u2010coding RNA H19. Importantly, an obvious H3K27ac enrichment is found at\n                    <jats:italic>Ctcf<\/jats:italic>\n                    , a trans\u2010activator of H19, and H3K27me3 enrichment at the H19 imprinting control region (ICR), suggesting that EED regulates H19 in an H3K27me3\u2010dependent manner. Intriguingly, H19 deletion reduces EED expression and the reprogramming of EED\u2010mediated H3K27me3 profiles, resulting in increased proliferation, differentiation, and decreased apoptosis of GCPs. Finally, molecular and genetic evidence provides that increased H19 expression is responsible for cerebellar hypoplasia and motor defects in EED mutant mice. Thus, this study demonstrates that EED, H19 forms a negative feedback loop, which plays a crucial role in cerebellar morphogenesis and controls cerebellar development.\n                  <\/jats:p>","DOI":"10.1002\/advs.202403591","type":"journal-article","created":{"date-parts":[[2024,11,5]],"date-time":"2024-11-05T06:49:14Z","timestamp":1730789354000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["An EED\/PRC2\u2010H19 Loop Regulates Cerebellar Development"],"prefix":"10.1002","volume":"12","author":[{"given":"Pei\u2010Pei","family":"Liu","sequence":"first","affiliation":[{"name":"Key Laboratory of Organ Regeneration and Reconstruction State Key Laboratory of Stem Cell and Reproductive Biology Institute of Zoology Chinese Academy of Sciences  Beijing 100101 China"},{"name":"University of Chinese Academy of Sciences  Beijing 100049 China"},{"name":"Institute for Stem Cell and Regeneration Chinese Academy of 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