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Here, a convenient and universal homogeneous detection platform named proximity\u2010unlocked luminescence by sequential enzymatic reactions from antibody and antibody\/aptamer (PULSERAA) is developed. This is designed so that the signal appears only when the donor and acceptor are in proximity on the viral surface. PULSERAA specifically detected in the range of 25\u2013500 digital copies\/mL of inactivated SARS\u2010CoV\u20102 after simply mixing reagents; it is elucidated that the accumulation of chemical species in a limited space of the viral surface contributed to such high sensitivity. PULSERAA was quickly adapated to detect another virus variant\n                    <jats:styled-content>,<\/jats:styled-content>\n                    inactivated influenza A virus, and infectious SARS\u2010CoV\u20102 in a clinical sample. Furthermore, on\u2010site (direct, rapid, and portable) visualization of the inactivated SARS\u2010CoV\u20102\u2010containing spots by a conventional smartphone camera was achieved, demonstrating that PULSERAA can be a practical tool for preventing the next pandemic in the future.\n                  <\/jats:p>","DOI":"10.1002\/advs.202403871","type":"journal-article","created":{"date-parts":[[2024,9,24]],"date-time":"2024-09-24T11:18:27Z","timestamp":1727176707000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Proximity\u2010Unlocked Luminescence by Sequential Enzymatic Reactions from Antibody and Antibody\/Aptamer (PULSERAA): A Platform for Detection and Visualization of Virus\u2010Containing Spots"],"prefix":"10.1002","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-0005-9976","authenticated-orcid":false,"given":"Daimei","family":"Miura","sequence":"first","affiliation":[{"name":"Department of 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