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Especially, local administration of LNPs\u2010formulated mRNA can cause off\u2010target translation of mRNA in distal organs which can induce unintended adverse effects. With the hypothesis that large and rigid nanoparticles can be applied to enhance retention of nanoparticles at the injection site, a polyethyleneimine (PEI)\u2010coated porous silica nanoparticles (PPSNs)\u2010based mRNA delivery platform is designed. PPSNs not only facilitate localized translation of mRNA at the site of injection but also prolonged protein expression. It is further demonstrated that the development of a highly efficacious Zika virus (ZIKV) vaccine using mRNA encoding full\u2010length ZIKV pre\u2010membrane (prM) and envelope (E) protein delivered by PPSNs. The ZIKV prME mRNA\u2010loaded PPSNs vaccine elicits robust immune responses, including high levels of neutralizing antibodies and ZIKV E\u2010specific T cell responses in C57BL\/6 mice. Moreover, a single injection of prME\u2010PPSNs vaccine provided complete protection against the ZIKV challenge in mice.<\/jats:p>","DOI":"10.1002\/advs.202404590","type":"journal-article","created":{"date-parts":[[2024,7,16]],"date-time":"2024-07-16T01:39:26Z","timestamp":1721093966000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["A Single\u2010Dose mRNA Vaccine Employing Porous Silica Nanoparticles Induces Robust Immune Responses Against the Zika Virus"],"prefix":"10.1002","volume":"11","author":[{"given":"Hojeong","family":"Shin","sequence":"first","affiliation":[{"name":"Department of Chemistry Seoul National University  Seoul 08826 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seounghun","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Chemistry Seoul National University  Seoul 08826 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheolhee","family":"Won","sequence":"additional","affiliation":[{"name":"Institute of Biotherapeutics Convergence Technology Lemonex Inc. 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