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However, the mechanisms of communication between TAMs and anaplastic thyroid cancer stem cells (ATCSCs) remain largely unelucidated. Integrative analyses of single\u2010cell RNA sequencing, cytokine\/chemokine arrays, proteomics, and mRNA expression datasets are performed to reveal crosstalk between TAMs and ATCSCs and signaling pathways in ATCSCs. Subsequently, in vitro experiments are performed to validate the regulatory effects of key cytokines on ATCSC stemness. Last, xenogeneic orthotopic thyroid ATCSCs transplantation models are utilized to corroborate the regulatory effect of cytokines on stemness. CCL20 derived from THP\u20101\u2010M2 activates the IRAK\u20101\/NF\u2010\u03baB1\/2 signaling pathway in ATCSCs, thereby positively regulating stemness characteristics and upregulating CXCL5 secretion. ATCSCs not only exhibit autocrine CXCL5 participation in the regulation of stemness but also demonstrate paracrine CXCL5 activity to recruit THP\u20101\u2010M\u03c6 and maintain the M2 phenotype. CCL20 and CXCL5 are involved in the crosstalk between TAMs and ATCSCs. The CCL20\/CXCL5 axis plays a crucial role in the interaction between TAMs and ATCSCs, establishing a progressive tumor microenvironment.<\/jats:p>","DOI":"10.1002\/advs.202405399","type":"journal-article","created":{"date-parts":[[2025,3,17]],"date-time":"2025-03-17T02:40:36Z","timestamp":1742179236000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["CCL20\/CXCL5 Drives Crosstalk Between Anaplastic Thyroid Cancer Stem Cells and Tumor\u2010Associated Macrophages to Promote Tumor Progression"],"prefix":"10.1002","volume":"12","author":[{"given":"Qi","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Thyroid Surgery The First Hospital of China Medical University  Shenyang 110801 P. R. 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