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A new nanotag, Ru@U6\u2010Ru\/Pt NPs is constructed for dual\u2010mode platforms by integrating double\u2010layered ECL luminophores and the nanozyme using Zr\u2010MOF (UiO\u201066\u2010NH\n                    <jats:sub>2<\/jats:sub>\n                    ) as the carrier, which not only generates enhanced ECL and colorimetric signals but also provide greater stability than that of commonly used nanotags. Dual\u2010mode platforms are used within 15\u00a0min from the \u201csample in\u201d to the \u201cresult out\u201d steps, without nucleic acid amplification. The colorimetric mode allows the screening of MPXV with the visual limit of detection (\n                    <jats:italic>v<\/jats:italic>\n                    LOD) of 0.1 pM (6 \u00d7 10\n                    <jats:sup>8<\/jats:sup>\n                    copies \u00b5L\n                    <jats:sup>\u22121<\/jats:sup>\n                    ) and the ECL mode supports quantitative detection of MPXV with an LOD as low as 10 aM (6 copies\u00b7\u00b5L\n                    <jats:sup>\u22121<\/jats:sup>\n                    ), resulting in a broad sensing range of 60 to 3 \u00d7 10\n                    <jats:sup>11<\/jats:sup>\n                    copies\u00b7\u00b5L\n                    <jats:sup>\u22121<\/jats:sup>\n                    (10 orders of magnitude). Validation is conducted using 50 clinical samples, which is 100% concordant to those of quantitative polymerase chain reaction (qPCR), indicating that Ru@U6\u2010Ru\/Pt NPs\u2010based dual\u2010mode sensing platforms showed great promise as rapid, sensitive, and accurate tools for diagnosis of the nucleic acid of MPXV and other infectious pathogens.\n                  <\/jats:p>","DOI":"10.1002\/advs.202405848","type":"journal-article","created":{"date-parts":[[2024,8,9]],"date-time":"2024-08-09T07:27:32Z","timestamp":1723188452000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":101,"title":["Zr\u2010MOF Carrier\u2010Enhanced Dual\u2010Mode Biosensing Platforms for Rapid and Sensitive Diagnosis of Mpox"],"prefix":"10.1002","volume":"11","author":[{"given":"Huiyi","family":"Yang","sequence":"first","affiliation":[{"name":"Molecular Diagnosis and Treatment Center for Infectious Diseases Dermatology Hospital Southern Medical University  Guangzhou 510000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judun","family":"Zheng","sequence":"additional","affiliation":[{"name":"Molecular Diagnosis and Treatment Center for Infectious Diseases Dermatology Hospital Southern Medical University  Guangzhou 510000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute for Engineering Medicine Kunming Medical University  Kunming 650500 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingyan","family":"Lin","sequence":"additional","affiliation":[{"name":"National Clinical Research Center for Infectious Disease The Second Affiliated Hospital of Southern University of Science and Technology Shenzhen Third People's Hospital  Shenzhen 518000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingru","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute for Engineering Medicine Kunming Medical University  Kunming 650500 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lunjing","family":"Liu","sequence":"additional","affiliation":[{"name":"NHC Key Laboratory of Metabolic Cardiovascular Diseases Research Ningxia Key Laboratory of Vascular Injury and Repair Research Ningxia Medical University  Yinchuan 750004 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenjie","family":"Wu","sequence":"additional","affiliation":[{"name":"Institute for Engineering Medicine Kunming Medical University  Kunming 650500 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengli","family":"Zhang","sequence":"additional","affiliation":[{"name":"Molecular Diagnosis and Treatment Center for Infectious Diseases Dermatology Hospital Southern Medical University  Guangzhou 510000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingxia","family":"Zhang","sequence":"additional","affiliation":[{"name":"National Clinical Research Center for Infectious Disease The Second Affiliated Hospital of Southern University of Science and Technology Shenzhen Third People's Hospital  Shenzhen 518000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Fu","sequence":"additional","affiliation":[{"name":"NHC Key Laboratory of Metabolic Cardiovascular Diseases Research Ningxia Key Laboratory of Vascular Injury and Repair Research Ningxia Medical University  Yinchuan 750004 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Yang","sequence":"additional","affiliation":[{"name":"Molecular Diagnosis and Treatment Center for Infectious Diseases Dermatology Hospital Southern Medical University  Guangzhou 510000 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