{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:25:04Z","timestamp":1786976704495,"version":"build-2736575974"},"reference-count":36,"publisher":"Wiley","issue":"42","license":[{"start":{"date-parts":[[2024,9,13]],"date-time":"2024-09-13T00:00:00Z","timestamp":1726185600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFC2704902"],"award-info":[{"award-number":["2022YFC2704902"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32001021"],"award-info":[{"award-number":["32001021"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Emerging diagnostic scenarios, such as population surveillance by pooled testing and on\u2010site rapid diagnosis, highlight the importance of advanced microfluidic systems for in vitro diagnostics. However, the widespread adoption of microfluidic technology faces challenges due to the lack of standardized design paradigms, posing difficulties in managing macro\u2010micro fluidic interfaces, reagent storage, and complex macrofluidic operations. This paper introduces a novel modular\u2010based mesoscopic design paradigm, featuring a core \u201cneedle\u2010plug\/piston\u201d structure with versatile variants for complex fluidic operations. These structures can be easily coupled with various microfluidic platforms to achieve truly self\u2010contained microsystems. Incorporated into a \u201c3D extensible\u201d design architecture, the mesoscopic design meets the demands of function integration, macrofluid manipulations, and flexible throughputs for point\u2010of\u2010care nucleic acid testing. Using this approach, an ultra\u2010sensitive nucleic acid detection system is developed with a limit of detection of ten copies of SARS\u2010CoV\u20102 per mL. This system efficiently conducts large\u2010scale pooled testing from 50 pharyngeal swabs in a tube with an uncompromised sensitivity, enabling a truly \u201csample\u2010in\u2010answer\u2010out\u201d microsystem with exceptional performance.<\/jats:p>","DOI":"10.1002\/advs.202406076","type":"journal-article","created":{"date-parts":[[2024,9,13]],"date-time":"2024-09-13T11:30:24Z","timestamp":1726227024000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Needle\u2010Plug\/Piston\u2010Based Modular Mesoscopic Design Paradigm Coupled With Microfluidic Device for Point\u2010of\u2010Care Pooled Testing"],"prefix":"10.1002","volume":"11","author":[{"given":"Baobao","family":"Lin","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering Tsinghua University  Beijing 100084 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bao","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Tsinghua University  Beijing 100084 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wu","family":"Zeng","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Tsinghua University  Beijing 100084 China"},{"name":"Changping Laboratory  Beijing 102206 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yulan","family":"Zhao","sequence":"additional","affiliation":[{"name":"Changping Laboratory  Beijing 102206 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huiping","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Tsinghua University  Beijing 100084 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yin","family":"Gu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Space Medicine China Astronaut Research and Training Center  Beijing 100094 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3000-3168","authenticated-orcid":false,"given":"Peng","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Tsinghua University  Beijing 100084 China"},{"name":"Changping Laboratory  Beijing 102206 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,9,13]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2404-8"},{"key":"e_1_2_8_1_2","first-page":"7326","volume":"3","author":"Parihar A.","year":"2020","journal-title":"Appl. Phys. Rev."},{"key":"e_1_2_8_1_3","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.adj8104"},{"key":"e_1_2_8_1_4","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2008-3"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-24552-4"},{"key":"e_1_2_8_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-18611-5"},{"key":"e_1_2_8_2_3","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciaa531"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41551-021-00754-5"},{"key":"e_1_2_8_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2885-5"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41551-020-00654-0"},{"key":"e_1_2_8_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41551-021-00833-7"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciaa1178"},{"key":"e_1_2_8_5_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abo0881"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bioeng.4.112601.125916"},{"key":"e_1_2_8_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2003.09.019"},{"key":"e_1_2_8_6_3","doi-asserted-by":"publisher","DOI":"10.1038\/nature05058"},{"key":"e_1_2_8_6_4","doi-asserted-by":"publisher","DOI":"10.1002\/smll.201000946"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1039\/b924109d"},{"key":"e_1_2_8_7_2","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2005.053348"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-019-1491-x"},{"key":"e_1_2_8_8_2","doi-asserted-by":"publisher","DOI":"10.1063\/1.1504171"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms6427"},{"key":"e_1_2_8_9_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aba0606"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01487-06"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-022-05408-3"},{"key":"e_1_2_8_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2022.116826"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1080\/14737159.2017.1275962"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1039\/c3lc50404b"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/mi10120873"},{"key":"e_1_2_8_14_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.0c00818"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac202028g"},{"key":"e_1_2_8_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2633"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2013.046"},{"key":"e_1_2_8_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2018.10.020"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.9b01560"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2022.114816"}],"container-title":["Advanced Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.202406076","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T15:20:47Z","timestamp":1759764047000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/advs.202406076"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9,13]]},"references-count":36,"journal-issue":{"issue":"42","published-print":{"date-parts":[[2024,11]]}},"alternative-id":["10.1002\/advs.202406076"],"URL":"https:\/\/doi.org\/10.1002\/advs.202406076","archive":["Portico"],"relation":{},"ISSN":["2198-3844","2198-3844"],"issn-type":[{"value":"2198-3844","type":"print"},{"value":"2198-3844","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,9,13]]},"assertion":[{"value":"2024-06-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-09-13","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2406076"}}