{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T13:11:00Z","timestamp":1790341860077,"version":"4.1.0"},"reference-count":55,"publisher":"Wiley","issue":"42","license":[{"start":{"date-parts":[[2024,9,19]],"date-time":"2024-09-19T00:00:00Z","timestamp":1726704000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22234006"],"award-info":[{"award-number":["22234006"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The colloidal gold nanoparticle (AuNP)\u2010based colorimetric lateral flow assay (LFA) is one of the most promising analytical tools for point\u2010of\u2010care disease diagnosis. However, the low sensitivity and insufficient accuracy still limit its clinical application. In this work, a machine learning (ML)\u2010optimized colorimetric LFA with ultrasound enrichment is developed to achieve the sensitive and accurate detection of tau proteins for early screening of Alzheimer's disease (AD). The LFA device is integrated with a portable ultrasonic actuator to rapidly enrich microparticles using ultrasound, which is essential for sample pre\u2010enrichment to improve the sensitivity, followed by ML algorithms to classify and predict the enhanced colorimetric signals. The results of the undiluted serum sample testing show that the protocol enables efficient classification and accurate quantification of the AD biomarker tau protein concentration with an average classification accuracy of 98.11% and an average prediction accuracy of 99.99%, achieving a limit of detection (LOD) as sensitive as 10.30\u00a0pg\u00a0mL\n                    <jats:sup>\u22121<\/jats:sup>\n                    . Further point\u2010of\u2010care testing (POCT) of human plasma samples demonstrates the potential use of LFA in clinical trials. Such a reliable lateral flow immunosensor with high precision and superb sensing performance is expected to put LFA in perspective as an AD clinical diagnostic platform.\n                  <\/jats:p>","DOI":"10.1002\/advs.202406196","type":"journal-article","created":{"date-parts":[[2024,9,19]],"date-time":"2024-09-19T05:46:01Z","timestamp":1726724761000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":47,"title":["Integrated Ultrasound\u2010Enrichment and Machine Learning in Colorimetric Lateral Flow Assay for Accurate and Sensitive Clinical Alzheimer's Biomarker Diagnosis"],"prefix":"10.1002","volume":"11","author":[{"given":"Shuqing","family":"Wang","sequence":"first","affiliation":[{"name":"School of Biomedical Engineering College of Chemistry and Environmental Engineering The Institute for Advanced Study (IAS) Shenzhen University  Shenzhen Guangdong 518060 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Zhu","sequence":"additional","affiliation":[{"name":"School of Biomedical Engineering College of Chemistry and Environmental Engineering The Institute for Advanced Study (IAS) Shenzhen University  Shenzhen Guangdong 518060 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongzeng","family":"Zhou","sequence":"additional","affiliation":[{"name":"School of Biomedical Engineering College of Chemistry and Environmental Engineering The Institute for Advanced Study (IAS) Shenzhen University  Shenzhen Guangdong 518060 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Luo","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory for Bioengineering and Sensing Technology University of Science and Technology Beijing  Beijing 100083 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Huang","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory for Bioengineering and Sensing Technology University of Science and Technology Beijing  Beijing 100083 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yibiao","family":"Liu","sequence":"additional","affiliation":[{"name":"Longgang District Central Hospital of Shenzhen  Shenzhen Guangdong 518116 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4037-2856","authenticated-orcid":false,"given":"Tailin","family":"Xu","sequence":"additional","affiliation":[{"name":"School of Biomedical Engineering College of Chemistry and Environmental Engineering The Institute for Advanced Study (IAS) Shenzhen University  Shenzhen Guangdong 518060 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,9,19]]},"reference":[{"key":"e_1_2_8_1_1","volume":"390","author":"Chin N. A.","year":"2024","journal-title":"N. Engl. J. Med."},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-023-02477-3"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/bios12080564"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202308783"},{"key":"e_1_2_8_5_1","first-page":"635","volume":"18","author":"Harten A. C.","year":"2022","journal-title":"Dementia"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2023.117174"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jalz.2011.03.005"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature25456"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2022.114278"},{"key":"e_1_2_8_10_1","first-page":"2614","volume":"18","author":"Moscoso A.","year":"2022","journal-title":"Dementia"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1039\/D1CS00127B"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.1c02149"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2022.114411"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202103458"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2020.104529"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nantod.2019.100831"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12951-021-01111-z"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1039\/D2AN00717G"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.9b06571"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2022.114094"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.42364"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202215548"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.28416"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.67184"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/smtd.202000924"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2022.116754"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-020-0931-3"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-35517-6"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-44186-y"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1039\/D1LC00628B"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms13068"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac3034536"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules24152759"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00604-017-2464-0"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2014.01.015"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.0c05032"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.0c01011"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2020.112185"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2021.122517"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2022.114903"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.2c05381"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2023.115746"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1088\/0957-4484\/19\/43\/435703"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12010-009-8535-x"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.201606039"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1039\/B601326K"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1002\/smll.201403621"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.201808247"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41576-019-0122-6"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1109\/TFUZZ.2018.2874017"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.1c00022"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.3c04441"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.3390\/bios7020023"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.3c10543"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1039\/D0CS00107D"}],"container-title":["Advanced Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.202406196","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T15:20:49Z","timestamp":1759764049000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/advs.202406196"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9,19]]},"references-count":55,"journal-issue":{"issue":"42","published-print":{"date-parts":[[2024,11]]}},"alternative-id":["10.1002\/advs.202406196"],"URL":"https:\/\/doi.org\/10.1002\/advs.202406196","archive":["Portico"],"relation":{},"ISSN":["2198-3844","2198-3844"],"issn-type":[{"value":"2198-3844","type":"print"},{"value":"2198-3844","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,9,19]]},"assertion":[{"value":"2024-06-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-09-19","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2406196"}}