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Cx26 is mainly expressed in supporting cells (SCs) and fibrocytes in the mammalian cochlea. Gene therapy is currently considered the most promising strategy for eradicating genetic diseases. However, there have been no significant effects of gene therapy for\n                    <jats:italic>GJB2<\/jats:italic>\n                    gene mutation\u2010associated deafness because deficiency of Cx26 leads to expanded sensory epithelial damage. In this study, the AAV2.7m8 serotype combined with the gfaABC1D promoter targeted infection of SCs is identified. It is found that\n                    <jats:italic>Gjb2<\/jats:italic>\n                    gene replacement therapy in wild\u2010type mice results in sensory hair cells (HCs) deficits, excessive inflammatory responses, and hearing loss. This may be one of the key factors contributing to the hardship of\n                    <jats:italic>GJB2<\/jats:italic>\n                    gene replacement therapy. Dexamethasone (DEX) shows promising results in inhibiting macrophage recruitment, with a protective effect against HC damage. Further, the combination of AAV2.7m8\u2010\n                    <jats:italic>Gjb2<\/jats:italic>\n                    with DEX shows a synergistic effect and enhances the gene therapy effect in a conditional Cx26 null mice model. These results indicate that the combination of gene therapy and medication will provide a new strategy for the treatment of hereditary deafness associated with\n                    <jats:italic>GJB2<\/jats:italic>\n                    defects.\n                  <\/jats:p>","DOI":"10.1002\/advs.202406510","type":"journal-article","created":{"date-parts":[[2024,12,30]],"date-time":"2024-12-30T05:35:48Z","timestamp":1735536948000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Viral\u2010Mediated Connexin 26 Expression Combined with Dexamethasone Rescues Hearing in a Conditional\n                    <i>Gjb2<\/i>\n                    Null Mice Model"],"prefix":"10.1002","volume":"12","author":[{"given":"Xiaohui","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Otorhinolaryngology Union Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan 430022 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology Union Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan 430022 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sen","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology Union Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan 430022 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Le","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology Union Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan 430022 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Qiu","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology Union Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan 430022 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenyang","family":"Kong","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology Union Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan 430022 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ge","family":"Yin","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology Union Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan 430022 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5410-2297","authenticated-orcid":false,"given":"Weijia","family":"Kong","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology Union Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan 430022 China"},{"name":"Institute of Otorhinolaryngology Tongji Medical College Huazhong University of Science and Technology  Wuhan 430022 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1771-3715","authenticated-orcid":false,"given":"Yu","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology Union Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan 430022 China"},{"name":"Institute of Otorhinolaryngology Tongji Medical College Huazhong University of Science and Technology  Wuhan 430022 China"},{"name":"Hubei Province Key Laboratory of Oral and Maxillofacial Development and Regeneration  Wuhan 430022 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,12,30]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(05)71047-3"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/cpr.13620"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-022-00938-8"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202304551"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-023-04743-6"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.engreg.2023.04.003"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-11687-8"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.8493530"},{"key":"e_1_2_9_9_1","first-page":"37","volume":"12","author":"Yin G.","year":"2023","journal-title":"Am. 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